TY - JOUR
T1 - Microwell array-mediated delivery of lipoplexes containing nucleic acids for enhanced therapeutic efficacy
AU - Wu, Yun
AU - Gallego-Perez, Daniel
AU - James Lee, L.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
PY - 2015
Y1 - 2015
N2 - Many delivery methods have been developed to improve the therapeutic efficacy and facilitate the clinical translation of nucleic acids-based therapeutics. We present a facile microwell array to mediate the delivery of nucleic acids carried by lipoplexes, which combines the advantages of lipoplexes as an efficient carrier system, the surface mediated delivery, and the control of surface topography. This method shows much higher transfection efficiency than conventional transfection method for oligodeoxynucleotides and microRNAs, and thus significantly reduces the effective therapeutic dosages. Microwell array is also a very flexible platform. Multifunctional lipoplexes containing both nucleic acid therapeutics and imaging reagents can be easily prepared in the microwell array and efficiently delivered to cells, demonstrating its potential applications in theranostic medicine.
AB - Many delivery methods have been developed to improve the therapeutic efficacy and facilitate the clinical translation of nucleic acids-based therapeutics. We present a facile microwell array to mediate the delivery of nucleic acids carried by lipoplexes, which combines the advantages of lipoplexes as an efficient carrier system, the surface mediated delivery, and the control of surface topography. This method shows much higher transfection efficiency than conventional transfection method for oligodeoxynucleotides and microRNAs, and thus significantly reduces the effective therapeutic dosages. Microwell array is also a very flexible platform. Multifunctional lipoplexes containing both nucleic acid therapeutics and imaging reagents can be easily prepared in the microwell array and efficiently delivered to cells, demonstrating its potential applications in theranostic medicine.
KW - Lipoplexes
KW - Microwell array
KW - Nucleic acid delivery
KW - Surface medicated delivery
KW - Theranostic medicine
UR - http://www.scopus.com/inward/record.url?scp=84955382725&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-1538-5_8
DO - 10.1007/978-1-4939-1538-5_8
M3 - Article
C2 - 25319649
AN - SCOPUS:84955382725
SN - 1064-3745
VL - 1218
SP - 131
EP - 142
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -