Micropatterns of cell adhesive proteins with poly(ethylene oxide)-block-Poly(4-vinylpyridine) diblock copolymer

J. Racine, H. Cheradame, Y. S. Chu, J. P. Thiery, I. Rodriguez*

*Corresponding author for this work

Research output: Contribution to journalLetterpeer-review

5 Scopus citations

Abstract

Control of cell shape and behavior through the micropattern technique by spatial immobilization of adhesive proteins on a surface has provided novel insights in several aspects of cell biology, such as tissue morphogenesis, cell growth and cell differentiation, and apoptosis. In this work, we present the use of poly(ethylene oxide-block-poly(4-vinylpyridine) (PEO-b-P4VP) as a non-adhesive background to construct micropatterns of cell adhesive proteins. In the method presented, PEO-b-P4VP is used for its antifouling properties and at the same time, as a photosensitive material to define the micropatterns. The irradiation of PEO-b-P4VP with a short wavelength UV light through photolithographic mask, causes the polymer to crosslink and immobilize in the areas exposed. In the areas non-exposed the polymer can be removed. These areas can be subsequent back filled with the adhesive protein of interest to produce the final micropatterned cell chips.

Original languageEnglish
Pages (from-to)983-987
Number of pages5
JournalBiotechnology and Bioengineering
Volume108
Issue number4
DOIs
StatePublished - Apr 2011

Keywords

  • Cell adhesion
  • Cell micropatterning
  • PEO-b-P4VP
  • Poly(ethylene oxide)-poly(4-vinylpyridine)

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