Microfluidic Enzyme-Linked Immunosorbent Assay Technology

L. James Lee*, Shang Tiang Yang, Siyi Lai, Yunling Bai, Wei Cho Huang, Yi Je Juang

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

30 Scopus citations


In this chapter, we have presented an overview of microfluidic enzyme-linked immunosorbent assay (ELISA) by first introducing the principle of immunoassay, ELISA, and microfabricated devices, followed by a discussion of microfabrication technology and the characterization of microfluidic components. Significant advances in laboratory technology are contributing to the further understanding of microfluidic function, surface modification and immobilization, which lead to the development of improved biomolecule detection methods and prospective applications. For the future, the exploitation of more robust-manufacturing processes and integrated assay systems in an automatic fashion with much reduced assay time and reagent consumption will allow for the effective detection and quantification of biological agents that are of interest in medical diagnostics, food safety surveillance, and environmental monitoring.

Original languageEnglish
Title of host publicationAdvances in Clinical Chemistry
EditorsGregory Makowski
Number of pages41
StatePublished - 2006

Publication series

NameAdvances in Clinical Chemistry
ISSN (Print)0065-2423


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