Micro-spectroscopy of chloroplasts in protoplasts from Arabidopsis thaliana under single- and multi-photon excitations

Fu Jen Kao*, Yi Min Wang, Jian Cheng Chen, Ping Chin Cheng, Rung Wu Chen, Bai Ling Lin

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

7 Scopus citations

Abstract

Ultrafast laser pulses are known to generate impacts on specimens in ways much different from cw illumination in the applications of confocal microscopy. In this study, protoplasts from Arabidopsis thaliana are employed as the samples to compare the effects induced by single- and multi-photon excitations under confocal imaging configuration. Through time-lapsed micro-spectroscopy we are able to characterize the differences in photo-bleaching and the evolution of fluorescence spectra of chloroplasts thus induced. These differences may be attributed to the disruption of chained reaction as a result of multi-photon excitation in photosynthesis.

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalJournal of Luminescence
Volume98
Issue number1-4
DOIs
StatePublished - Jul 2002
EventHBSM 2001 - Taipei, Taiwan
Duration: 18 Nov 200123 Nov 2001

Keywords

  • Chloroplasts
  • Micro-spectroscopy
  • Multi-photon excitation
  • Photo-bleaching

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