Micro-/nanoscale electroporation

Lingqian Chang; Lei Li; Junfeng Shi; Yan Sheng; Wu Lu; Daniel Gallego-Perez; Ly James Lee

doi:10.1039/c6lc00840b

Micro-/nanoscale electroporation

Lingqian Chang, Lei Li, Junfeng Shi, Yan Sheng, Wu Lu, Daniel Gallego-Perez^*, Ly James Lee

^*Corresponding author for this work

Institute of Biopharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

91 Scopus citations

Abstract

Electroporation has been one of the most popular non-viral technologies for cell transfection. However, conventional bulk electroporation (BEP) shows significant limitations in efficiency, cell viability and transfection uniformity. Recent advances in microscale-electroporation (MEP) resulted in improved cell viability. Further miniaturization of the electroporation system (i.e., nanoscale) has brought up many unique advantages, including negligible cell damage and dosage control capabilities with single-cell resolution, which has enabled more translational applications. In this review, we give an insight into the fundamental and technical aspects of micro- and nanoscale/nanochannel electroporation (NEP) and go over several examples of MEP/NEP-based cutting-edge research, including gene editing, adoptive immunotherapy, and cellular reprogramming. The challenges and opportunities of advanced electroporation technologies are also discussed.

Original language	English
Pages (from-to)	4047-4062
Number of pages	16
Journal	Lab on a Chip
Volume	16
Issue number	21
DOIs	https://doi.org/10.1039/c6lc00840b
State	Published - 2016

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title = "Micro-/nanoscale electroporation",

abstract = "Electroporation has been one of the most popular non-viral technologies for cell transfection. However, conventional bulk electroporation (BEP) shows significant limitations in efficiency, cell viability and transfection uniformity. Recent advances in microscale-electroporation (MEP) resulted in improved cell viability. Further miniaturization of the electroporation system (i.e., nanoscale) has brought up many unique advantages, including negligible cell damage and dosage control capabilities with single-cell resolution, which has enabled more translational applications. In this review, we give an insight into the fundamental and technical aspects of micro- and nanoscale/nanochannel electroporation (NEP) and go over several examples of MEP/NEP-based cutting-edge research, including gene editing, adoptive immunotherapy, and cellular reprogramming. The challenges and opportunities of advanced electroporation technologies are also discussed.",

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T1 - Micro-/nanoscale electroporation

AU - Chang, Lingqian

AU - Li, Lei

AU - Shi, Junfeng

AU - Sheng, Yan

AU - Lu, Wu

AU - Gallego-Perez, Daniel

AU - Lee, Ly James

N1 - Publisher Copyright: © The Royal Society of Chemistry 2016.

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Y1 - 2016

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AB - Electroporation has been one of the most popular non-viral technologies for cell transfection. However, conventional bulk electroporation (BEP) shows significant limitations in efficiency, cell viability and transfection uniformity. Recent advances in microscale-electroporation (MEP) resulted in improved cell viability. Further miniaturization of the electroporation system (i.e., nanoscale) has brought up many unique advantages, including negligible cell damage and dosage control capabilities with single-cell resolution, which has enabled more translational applications. In this review, we give an insight into the fundamental and technical aspects of micro- and nanoscale/nanochannel electroporation (NEP) and go over several examples of MEP/NEP-based cutting-edge research, including gene editing, adoptive immunotherapy, and cellular reprogramming. The challenges and opportunities of advanced electroporation technologies are also discussed.

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Micro-/nanoscale electroporation

Abstract

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