Micro-flow-cytometer integrated with optical tweezer and DIP technique for cell/particle sorting and manipulation

Yu Sheng Chien*, Che Hsin Lin, Fu Jen Kao, Cheng Wen Ko

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

This paper proposes a novel microfluidic system for cell/microparticle recognition and manipulation utilizing digital image processing technique (DIP) and optical tweezer under microfluidic configuration. Digital image processing technique is used to count and recognize the cell/particle samples and then sends a control signal to generate a laser pulse to manipulate the target cell/particle optically. The optical tweezer system is capable of catching, moving and switching the target cells at the downstream of the microchannel. The trapping force of the optical tweezer is also demonstrated utilizing Stocks-drag method and electroosmotic flow. The proposed system provides a simple but high-performance solution for microparticle manipulation in a microfluidic device.

Original languageEnglish
Title of host publicationNanoscience and Technology
PublisherTrans Tech Publications Ltd.
Pages1351-1354
Number of pages4
EditionPART 2
ISBN (Print)3908451302, 9783908451303
DOIs
StatePublished - 2007
EventChina International Conference on Nanoscience and Technology, ChinaNANO 2005 - Beijing, China
Duration: 9 Jun 200511 Jun 2005

Publication series

NameSolid State Phenomena
NumberPART 2
Volume121-123
ISSN (Print)1012-0394

Conference

ConferenceChina International Conference on Nanoscience and Technology, ChinaNANO 2005
Country/TerritoryChina
CityBeijing
Period9/06/0511/06/05

Keywords

  • Cell manipulation
  • Digital image processing
  • Flow cytometer
  • Optical tweezer

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