Meta-lens light-sheet fluorescence microscopy for in vivo imaging

Yuan Luo; Ming Lun Tseng; Sunil Vyas; Ting Yu Hsieh; Jui Ching Wu; Shang Yang Chen; Hsiao Fang Peng; Vin Cent Su; Tzu Ting Huang; Hsin Yu Kuo; Cheng Hung Chu; Mu Ku Chen; Jia Wern Chen; Yu Chun Chen; Kuang Yuh Huang; Chieh Hsiung Kuan; Xu Shi; Hiroaki Misawa; Din Ping Tsai

doi:10.1515/nanoph-2021-0748

Meta-lens light-sheet fluorescence microscopy for in vivo imaging

Yuan Luo^*, Ming Lun Tseng, Sunil Vyas, Ting Yu Hsieh, Jui Ching Wu^*, Shang Yang Chen, Hsiao Fang Peng, Vin Cent Su, Tzu Ting Huang, Hsin Yu Kuo, Cheng Hung Chu, Mu Ku Chen, Jia Wern Chen, Yu Chun Chen, Kuang Yuh Huang, Chieh Hsiung Kuan, Xu Shi, Hiroaki Misawa, Din Ping Tsai^*

^*Corresponding author for this work

Institute of Electronics

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution.

Original language	English
Pages (from-to)	1949-1959
Number of pages	11
Journal	Nanophotonics
Volume	11
Issue number	9
DOIs	https://doi.org/10.1515/nanoph-2021-0748
State	Published - 1 Apr 2022

Keywords

fluorescence microscopy
light sheet microscopy
metasurface

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10.1515/nanoph-2021-0748

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Luo, Y., Tseng, M. L., Vyas, S., Hsieh, T. Y., Wu, J. C., Chen, S. Y., Peng, H. F., Su, V. C., Huang, T. T., Kuo, H. Y., Chu, C. H., Chen, M. K., Chen, J. W., Chen, Y. C., Huang, K. Y., Kuan, C. H., Shi, X., Misawa, H., & Tsai, D. P. (2022). Meta-lens light-sheet fluorescence microscopy for in vivo imaging. Nanophotonics, 11(9), 1949-1959. https://doi.org/10.1515/nanoph-2021-0748

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title = "Meta-lens light-sheet fluorescence microscopy for in vivo imaging",

abstract = "Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution.",

keywords = "fluorescence microscopy, light sheet microscopy, metasurface",

author = "Yuan Luo and Tseng, {Ming Lun} and Sunil Vyas and Hsieh, {Ting Yu} and Wu, {Jui Ching} and Chen, {Shang Yang} and Peng, {Hsiao Fang} and Su, {Vin Cent} and Huang, {Tzu Ting} and Kuo, {Hsin Yu} and Chu, {Cheng Hung} and Chen, {Mu Ku} and Chen, {Jia Wern} and Chen, {Yu Chun} and Huang, {Kuang Yuh} and Kuan, {Chieh Hsiung} and Xu Shi and Hiroaki Misawa and Tsai, {Din Ping}",

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doi = "10.1515/nanoph-2021-0748",

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AU - Chen, Shang Yang

AU - Peng, Hsiao Fang

AU - Su, Vin Cent

AU - Huang, Tzu Ting

AU - Kuo, Hsin Yu

AU - Chu, Cheng Hung

AU - Chen, Mu Ku

AU - Chen, Jia Wern

AU - Chen, Yu Chun

AU - Huang, Kuang Yuh

AU - Kuan, Chieh Hsiung

AU - Shi, Xu

AU - Misawa, Hiroaki

AU - Tsai, Din Ping

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N2 - Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution.

AB - Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution.

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Meta-lens light-sheet fluorescence microscopy for in vivo imaging

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