Meta-lens light-sheet fluorescence microscopy for in vivo imaging

Yuan Luo*, Ming Lun Tseng, Sunil Vyas, Ting Yu Hsieh, Jui Ching Wu, Shang Yang Chen, Hsiao Fang Peng, Vin Cent Su, Tzu Ting Huang, Hsin Yu Kuo, Cheng Hung Chu, Mu Ku Chen, Jia Wern Chen, Yu Chun Chen, Kuang Yuh Huang, Chieh Hsiung Kuan, Xu Shi, Hiroaki Misawa, Din Ping Tsai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution.

Original languageEnglish
JournalNanophotonics
DOIs
StateAccepted/In press - 2022

Keywords

  • fluorescence microscopy
  • light sheet microscopy
  • metasurface

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