Low noise, self-phase-modulation-enabled femtosecond fiber sources tunable in 740-1236 nm for wide two-photon fluorescence microscopy applications

LU TING CHOU; YU CHENG LIU; DONG LIN ZHONG; WEI ZHONG LIN; HAO HSUAN HUNG; CHAO JIN CHAN; ZI PING CHEN; SHIH HSUAN CHIA

doi:10.1364/BOE.422668

Low noise, self-phase-modulation-enabled femtosecond fiber sources tunable in 740-1236 nm for wide two-photon fluorescence microscopy applications

LU TING CHOU, YU CHENG LIU, DONG LIN ZHONG, WEI ZHONG LIN, HAO HSUAN HUNG, CHAO JIN CHAN, ZI PING CHEN, SHIH HSUAN CHIA^*

^*Corresponding author for this work

Institute of Biophotonics

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We have demonstrated widely tunable Yb:fiber-based laser sources, aiming to replace Ti:sapphire lasers for the nJ-level ultrafast applications, especially for the uses of nonlinear light microscopy. We investigated the influence of different input parameters to obtain an expansive spectral broadening, enabled by self-phase modulation and further reshaped by self-steepening, in the normal dispersion regime before the fiber damage. We also discussed the compressibility and intensity fluctuations of the demonstrated pulses, to reach the transform-limited duration with a very low intensity noise. Most importantly, we have demonstrated clear two-photon fluorescence images from UV-absorbing fluorophores to deep red dye stains.

Original language	English
Pages (from-to)	2888-2901
Number of pages	14
Journal	Biomedical Optics Express
Volume	12
Issue number	5
DOIs	https://doi.org/10.1364/BOE.422668
State	Published - 1 May 2021

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CHOU, LU. TING., LIU, YU. CHENG., ZHONG, DONG. LIN., LIN, WEI. ZHONG., HUNG, HAO. HSUAN., CHAN, CHAO. JIN., CHEN, ZI. PING., & CHIA, SHIH. HSUAN. (2021). Low noise, self-phase-modulation-enabled femtosecond fiber sources tunable in 740-1236 nm for wide two-photon fluorescence microscopy applications. Biomedical Optics Express, 12(5), 2888-2901. https://doi.org/10.1364/BOE.422668

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title = "Low noise, self-phase-modulation-enabled femtosecond fiber sources tunable in 740-1236 nm for wide two-photon fluorescence microscopy applications",

abstract = "We have demonstrated widely tunable Yb:fiber-based laser sources, aiming to replace Ti:sapphire lasers for the nJ-level ultrafast applications, especially for the uses of nonlinear light microscopy. We investigated the influence of different input parameters to obtain an expansive spectral broadening, enabled by self-phase modulation and further reshaped by self-steepening, in the normal dispersion regime before the fiber damage. We also discussed the compressibility and intensity fluctuations of the demonstrated pulses, to reach the transform-limited duration with a very low intensity noise. Most importantly, we have demonstrated clear two-photon fluorescence images from UV-absorbing fluorophores to deep red dye stains. ",

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doi = "10.1364/BOE.422668",

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AU - CHOU, LU TING

AU - LIU, YU CHENG

AU - ZHONG, DONG LIN

AU - LIN, WEI ZHONG

AU - HUNG, HAO HSUAN

AU - CHAN, CHAO JIN

AU - CHEN, ZI PING

AU - CHIA, SHIH HSUAN

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AB - We have demonstrated widely tunable Yb:fiber-based laser sources, aiming to replace Ti:sapphire lasers for the nJ-level ultrafast applications, especially for the uses of nonlinear light microscopy. We investigated the influence of different input parameters to obtain an expansive spectral broadening, enabled by self-phase modulation and further reshaped by self-steepening, in the normal dispersion regime before the fiber damage. We also discussed the compressibility and intensity fluctuations of the demonstrated pulses, to reach the transform-limited duration with a very low intensity noise. Most importantly, we have demonstrated clear two-photon fluorescence images from UV-absorbing fluorophores to deep red dye stains.

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Low noise, self-phase-modulation-enabled femtosecond fiber sources tunable in 740-1236 nm for wide two-photon fluorescence microscopy applications

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