Low Intensity Ultrasound Induces Epithelial Cell Adhesion Responses

Jormay Lim; Yeh Shiu Chu; Ya Cherng Chu; Chun Min Lo; Jaw Lin Wang

doi:10.1115/1.4046883

Low Intensity Ultrasound Induces Epithelial Cell Adhesion Responses

Jormay Lim, Yeh Shiu Chu, Ya Cherng Chu, Chun Min Lo, Jaw Lin Wang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

In this study, we investigated the cellular mechanosensitive responses to a low intensity ultrasound (LIUS) stimulation (ISATA = 1 mW/cm2, pressure = 10 kPa). The dose and temporal effects at cell-substrate adhesion (CSA) at the basal level and cell-cell adhesion (CCA) at the apical level are reported in detail. A model of mouse mammary gland epithelial cells (EpH4) and the phosphorylation of mechanosensitive 130 kDa Crk-associated substrate (p130CAS) as an indicator for cellular responses were used. The intensity of phospho-p130CAS was found to be dependent on LIUS stress level, and the p130CAS was phosphorylated after 1 min stimulation at CSA. The phospho-p130CAS was also found to increase significantly at CCA upon LIUS stimulation. We confirmed that the cellular responses to ultrasound are immediate and dose dependent. Ultrasound affects not only CSA but also CCA. An E-cadherin knockout (EpH4ECad-/-) model also confirmed that phosphorylation of p130CAS at CCA is related to E-cadherins.

Original language	English
Article number	091014
Journal	Journal of Biomechanical Engineering
Volume	142
Issue number	9
DOIs	https://doi.org/10.1115/1.4046883
State	Published - 1 Sep 2020

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@article{73b0991b800c457ab09eb6dcc6cd4729,

title = "Low Intensity Ultrasound Induces Epithelial Cell Adhesion Responses",

abstract = "In this study, we investigated the cellular mechanosensitive responses to a low intensity ultrasound (LIUS) stimulation (ISATA = 1 mW/cm2, pressure = 10 kPa). The dose and temporal effects at cell-substrate adhesion (CSA) at the basal level and cell-cell adhesion (CCA) at the apical level are reported in detail. A model of mouse mammary gland epithelial cells (EpH4) and the phosphorylation of mechanosensitive 130 kDa Crk-associated substrate (p130CAS) as an indicator for cellular responses were used. The intensity of phospho-p130CAS was found to be dependent on LIUS stress level, and the p130CAS was phosphorylated after 1 min stimulation at CSA. The phospho-p130CAS was also found to increase significantly at CCA upon LIUS stimulation. We confirmed that the cellular responses to ultrasound are immediate and dose dependent. Ultrasound affects not only CSA but also CCA. An E-cadherin knockout (EpH4ECad-/-) model also confirmed that phosphorylation of p130CAS at CCA is related to E-cadherins.",

author = "Jormay Lim and Chu, {Yeh Shiu} and Chu, {Ya Cherng} and Lo, {Chun Min} and Wang, {Jaw Lin}",

year = "2020",

month = sep,

day = "1",

doi = "10.1115/1.4046883",

language = "English",

volume = "142",

journal = "Journal of Biomechanical Engineering",

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T1 - Low Intensity Ultrasound Induces Epithelial Cell Adhesion Responses

AU - Lim, Jormay

AU - Chu, Yeh Shiu

AU - Chu, Ya Cherng

AU - Lo, Chun Min

AU - Wang, Jaw Lin

PY - 2020/9/1

Y1 - 2020/9/1

N2 - In this study, we investigated the cellular mechanosensitive responses to a low intensity ultrasound (LIUS) stimulation (ISATA = 1 mW/cm2, pressure = 10 kPa). The dose and temporal effects at cell-substrate adhesion (CSA) at the basal level and cell-cell adhesion (CCA) at the apical level are reported in detail. A model of mouse mammary gland epithelial cells (EpH4) and the phosphorylation of mechanosensitive 130 kDa Crk-associated substrate (p130CAS) as an indicator for cellular responses were used. The intensity of phospho-p130CAS was found to be dependent on LIUS stress level, and the p130CAS was phosphorylated after 1 min stimulation at CSA. The phospho-p130CAS was also found to increase significantly at CCA upon LIUS stimulation. We confirmed that the cellular responses to ultrasound are immediate and dose dependent. Ultrasound affects not only CSA but also CCA. An E-cadherin knockout (EpH4ECad-/-) model also confirmed that phosphorylation of p130CAS at CCA is related to E-cadherins.

AB - In this study, we investigated the cellular mechanosensitive responses to a low intensity ultrasound (LIUS) stimulation (ISATA = 1 mW/cm2, pressure = 10 kPa). The dose and temporal effects at cell-substrate adhesion (CSA) at the basal level and cell-cell adhesion (CCA) at the apical level are reported in detail. A model of mouse mammary gland epithelial cells (EpH4) and the phosphorylation of mechanosensitive 130 kDa Crk-associated substrate (p130CAS) as an indicator for cellular responses were used. The intensity of phospho-p130CAS was found to be dependent on LIUS stress level, and the p130CAS was phosphorylated after 1 min stimulation at CSA. The phospho-p130CAS was also found to increase significantly at CCA upon LIUS stimulation. We confirmed that the cellular responses to ultrasound are immediate and dose dependent. Ultrasound affects not only CSA but also CCA. An E-cadherin knockout (EpH4ECad-/-) model also confirmed that phosphorylation of p130CAS at CCA is related to E-cadherins.

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DO - 10.1115/1.4046883

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SN - 0148-0731

VL - 142

JO - Journal of Biomechanical Engineering

JF - Journal of Biomechanical Engineering

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M1 - 091014

ER -

Low Intensity Ultrasound Induces Epithelial Cell Adhesion Responses

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