Long working distance fluorescence lifetime imaging with stimulated emission and electronic time delay

Po Yen Lin*, Shin Shian Lee, Chia Seng Chang, Fu Jen Kao

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

In this work, long working distance fluorescence lifetime imaging is realized with stimulated emission in combination with electronic time delay control. Spatial coherence, as a result of stimulated emission, supports unattenuated fluorescence detection at extended distance, using low NA optics. An electronic time delayed trigger provides an advantageous way in adjusting the pulse separation and probing the fluorescence lifetime in the nanosecond ranges. The fluorescence lifetime of selected fluorophores is accurately determined through the pump-probe configuration. The characteristics and applications in fluorescence lifetime measurement of stimulated emission are investigated and summarized succinctly here.

Original languageEnglish
Pages (from-to)11445-11450
Number of pages6
JournalOptics Express
Volume20
Issue number10
DOIs
StatePublished - 7 May 2012

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