Long-distance fluorescence lifetime imaging with stimulated emission and electronic time delay

Po Yen Lin*, Shin Shian Lee, Chia Seng Chang, Fu Jen Kao

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this work, long-distance fluorescence lifetime imaging is realized by stimulated emission and electronic time delay control. Spatial coherence, as a result of stimulated emission, provides unattenuated fluorescence detection at long distance with low NA optics. The electronic trigger also provides a feasible way to change the pulse separation and probe the fluorescence lifetime in nano-second range. The lifetime of fluorophores is determined by measuring the stimulated emission signal at various the time delays between excitation and stimulation pulses. The characteristics of stimulated emission are investigated and the saturation condition is also presented.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XII
DOIs
StatePublished - 2012
EventMultiphoton Microscopy in the Biomedical Sciences XII - San Francisco, CA, United States
Duration: 22 Jan 201224 Jan 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8226
ISSN (Print)1605-7422

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XII
Country/TerritoryUnited States
CitySan Francisco, CA
Period22/01/1224/01/12

Keywords

  • Fluorescence lifetime imaging
  • Long-distance fluorescence detection
  • stimulated emission imaging

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