Line-scanning detection instrument for photonic crystal enhanced fluorescence

Vikram Chaudhery; Meng Lu; Cheng-Sheng Huang; James Polans; Ruimin Tan; Richard C. Zangar; Brian T. Cunningham

doi:10.1364/OL.37.002565

Line-scanning detection instrument for photonic crystal enhanced fluorescence

Vikram Chaudhery, Meng Lu, Cheng-Sheng Huang, James Polans, Ruimin Tan, Richard C. Zangar, Brian T. Cunningham^*

^*Corresponding author for this work

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A laser line-scanning instrument was developed to optimize the near-field enhancement capability of a onedimensional photonic crystal (PC) for excitation of surface-bound fluorophores. The excitation laser beam is shaped into an 8 μm × 1 mm line that is focused along the direction of the PC grating, while remaining collimated perpendicular to the grating. Such a beam configuration offers high excitation power density while simultaneously providing high resonant coupling efficiency from the laser to the PC surface. Using a panel of 21 immunofluorescence assays on the PC surface in a microarray format, the approach achieves an enhancement factor as high as 90-fold between on-resonance and off-resonance illumination. The instrument provides a capability for sensitive and inexpensive analysis of cancer biomarkers in clinical applications.

Original language	English
Pages (from-to)	2565-2567
Number of pages	3
Journal	Optics Letters
Volume	37
Issue number	13
DOIs	https://doi.org/10.1364/OL.37.002565
State	Published - 1 Jul 2012

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abstract = "A laser line-scanning instrument was developed to optimize the near-field enhancement capability of a onedimensional photonic crystal (PC) for excitation of surface-bound fluorophores. The excitation laser beam is shaped into an 8 μm × 1 mm line that is focused along the direction of the PC grating, while remaining collimated perpendicular to the grating. Such a beam configuration offers high excitation power density while simultaneously providing high resonant coupling efficiency from the laser to the PC surface. Using a panel of 21 immunofluorescence assays on the PC surface in a microarray format, the approach achieves an enhancement factor as high as 90-fold between on-resonance and off-resonance illumination. The instrument provides a capability for sensitive and inexpensive analysis of cancer biomarkers in clinical applications.",

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AU - Chaudhery, Vikram

AU - Lu, Meng

AU - Huang, Cheng-Sheng

AU - Polans, James

AU - Tan, Ruimin

AU - Zangar, Richard C.

AU - Cunningham, Brian T.

PY - 2012/7/1

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AB - A laser line-scanning instrument was developed to optimize the near-field enhancement capability of a onedimensional photonic crystal (PC) for excitation of surface-bound fluorophores. The excitation laser beam is shaped into an 8 μm × 1 mm line that is focused along the direction of the PC grating, while remaining collimated perpendicular to the grating. Such a beam configuration offers high excitation power density while simultaneously providing high resonant coupling efficiency from the laser to the PC surface. Using a panel of 21 immunofluorescence assays on the PC surface in a microarray format, the approach achieves an enhancement factor as high as 90-fold between on-resonance and off-resonance illumination. The instrument provides a capability for sensitive and inexpensive analysis of cancer biomarkers in clinical applications.

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Line-scanning detection instrument for photonic crystal enhanced fluorescence

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