Laser irradiated growth of protein crystal

Hiroaki Adachi; Kazufumi Takano; Youichiroh Hosokawa; Tsuyoshi Inoue; Yusuke Mori; Hiroyoshi Matsumura; Masashi Yoshimura; Yasuo Tsunaka; Masaaki Morikawa; Shigenori Kanaya; Hiroshi Masuhara; Yasushi Kai; Takatomo Sasaki

doi:10.1143/JJAP.42.L798

Laser irradiated growth of protein crystal

Hiroaki Adachi^*, Kazufumi Takano, Youichiroh Hosokawa, Tsuyoshi Inoue, Yusuke Mori, Hiroyoshi Matsumura, Masashi Yoshimura, Yasuo Tsunaka, Masaaki Morikawa, Shigenori Kanaya, Hiroshi Masuhara, Yasushi Kai, Takatomo Sasaki

^*Corresponding author for this work

Department of Applied Chemistry

Research output: Contribution to journal › Letter › peer-review

133 Scopus citations

Abstract

We succeeded in the first ever generation of protein crystals by laser irradiation. We call this process Laser Irradiated Growth Technique (LIGHT). Effective crystallization was confirmed by applying an intense femtosecond laser. The crystallization period was dramatically shortened by LIGHT. In addition, protein crystals were obtained by LIGHT from normally uncrystallized conditions. These results indicate that intense femtosecond laser irradiation generates crystal nuclei; protein crystals can then be grown from the nuclei that act as seeds in a supersaturated solution. The nuclei formation is possible primarily due to nonlinear nucleation processes of an intense femtosecond laser with a peak intensity of over a gigawatt (GW).

Original language	English
Pages (from-to)	L798-L800
Number of pages	3
Journal	Japanese Journal of Applied Physics, Part 2: Letters
Volume	42
Issue number	7 B
DOIs	https://doi.org/10.1143/JJAP.42.L798
State	Published - Jul 2003

Keywords

Laser
Nucleation
Protein crystal
Short-pulse
Supersaturated solution

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10.1143/JJAP.42.L798

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title = "Laser irradiated growth of protein crystal",

abstract = "We succeeded in the first ever generation of protein crystals by laser irradiation. We call this process Laser Irradiated Growth Technique (LIGHT). Effective crystallization was confirmed by applying an intense femtosecond laser. The crystallization period was dramatically shortened by LIGHT. In addition, protein crystals were obtained by LIGHT from normally uncrystallized conditions. These results indicate that intense femtosecond laser irradiation generates crystal nuclei; protein crystals can then be grown from the nuclei that act as seeds in a supersaturated solution. The nuclei formation is possible primarily due to nonlinear nucleation processes of an intense femtosecond laser with a peak intensity of over a gigawatt (GW).",

keywords = "Laser, Nucleation, Protein crystal, Short-pulse, Supersaturated solution",

author = "Hiroaki Adachi and Kazufumi Takano and Youichiroh Hosokawa and Tsuyoshi Inoue and Yusuke Mori and Hiroyoshi Matsumura and Masashi Yoshimura and Yasuo Tsunaka and Masaaki Morikawa and Shigenori Kanaya and Hiroshi Masuhara and Yasushi Kai and Takatomo Sasaki",

year = "2003",

month = jul,

doi = "10.1143/JJAP.42.L798",

language = "English",

volume = "42",

pages = "L798--L800",

journal = "Japanese Journal of Applied Physics, Part 2: Letters",

issn = "0021-4922",

publisher = "Japan Society of Applied Physics",

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TY - JOUR

T1 - Laser irradiated growth of protein crystal

AU - Adachi, Hiroaki

AU - Takano, Kazufumi

AU - Hosokawa, Youichiroh

AU - Inoue, Tsuyoshi

AU - Mori, Yusuke

AU - Matsumura, Hiroyoshi

AU - Yoshimura, Masashi

AU - Tsunaka, Yasuo

AU - Morikawa, Masaaki

AU - Kanaya, Shigenori

AU - Masuhara, Hiroshi

AU - Kai, Yasushi

AU - Sasaki, Takatomo

PY - 2003/7

Y1 - 2003/7

N2 - We succeeded in the first ever generation of protein crystals by laser irradiation. We call this process Laser Irradiated Growth Technique (LIGHT). Effective crystallization was confirmed by applying an intense femtosecond laser. The crystallization period was dramatically shortened by LIGHT. In addition, protein crystals were obtained by LIGHT from normally uncrystallized conditions. These results indicate that intense femtosecond laser irradiation generates crystal nuclei; protein crystals can then be grown from the nuclei that act as seeds in a supersaturated solution. The nuclei formation is possible primarily due to nonlinear nucleation processes of an intense femtosecond laser with a peak intensity of over a gigawatt (GW).

AB - We succeeded in the first ever generation of protein crystals by laser irradiation. We call this process Laser Irradiated Growth Technique (LIGHT). Effective crystallization was confirmed by applying an intense femtosecond laser. The crystallization period was dramatically shortened by LIGHT. In addition, protein crystals were obtained by LIGHT from normally uncrystallized conditions. These results indicate that intense femtosecond laser irradiation generates crystal nuclei; protein crystals can then be grown from the nuclei that act as seeds in a supersaturated solution. The nuclei formation is possible primarily due to nonlinear nucleation processes of an intense femtosecond laser with a peak intensity of over a gigawatt (GW).

KW - Laser

KW - Nucleation

KW - Protein crystal

KW - Short-pulse

KW - Supersaturated solution

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U2 - 10.1143/JJAP.42.L798

DO - 10.1143/JJAP.42.L798

M3 - Letter

AN - SCOPUS:0141496372

SN - 0021-4922

VL - 42

SP - L798-L800

JO - Japanese Journal of Applied Physics, Part 2: Letters

JF - Japanese Journal of Applied Physics, Part 2: Letters

IS - 7 B

ER -

Laser irradiated growth of protein crystal

Abstract

Keywords

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