TY - JOUR
T1 - Laser-induced withdrawal test for electrophysiological recordings of nociception
AU - Kao, Yu Chieh
AU - Jaw, Fu Shan
N1 - Funding Information:
Acknowledgments This study was supported by NSC 97-2221-E-002-004-MY3. All experiments were carried out in accordance with the guidelines of the National Taiwan University College of Medicine and the College of Public Health Institutional Animal Care and Use Committee (IACUC) as well as the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 80-23). All efforts were made to minimize the number of animals required and to prevent any suffering.
PY - 2012/1
Y1 - 2012/1
N2 - CO 2 lasers are often used in pain research. However, the stimulation parameters of the CO 2 lasers, such as beam diameter, laser power, etc., used for these animal nociceptive studies vary across laboratories. The differences of the parameters usually make novices who want to reproduce the laser-evoked responses confused to follow. In this study, we quantitatively measured the laser-withdrawal reflex of the rat to ascertain the individual laser-withdrawal threshold and then found the thresholds were diverse among the rats we adopted. Furthermore, the optimal stimulation distance, the most conveniently modified stimulation parameter of CO 2 laser stimuli, was also determined. We suggest that laser-withdrawal tests should be applied before the electrophysiological recordings in order to verify the efficiency of the induced nociception and substantiate the operational parameters of the CO 2 laser device.
AB - CO 2 lasers are often used in pain research. However, the stimulation parameters of the CO 2 lasers, such as beam diameter, laser power, etc., used for these animal nociceptive studies vary across laboratories. The differences of the parameters usually make novices who want to reproduce the laser-evoked responses confused to follow. In this study, we quantitatively measured the laser-withdrawal reflex of the rat to ascertain the individual laser-withdrawal threshold and then found the thresholds were diverse among the rats we adopted. Furthermore, the optimal stimulation distance, the most conveniently modified stimulation parameter of CO 2 laser stimuli, was also determined. We suggest that laser-withdrawal tests should be applied before the electrophysiological recordings in order to verify the efficiency of the induced nociception and substantiate the operational parameters of the CO 2 laser device.
KW - CO lasers
KW - Hind paw
KW - Laser-withdrawal threshold
KW - Rat
KW - Stimulation parameter
UR - http://www.scopus.com/inward/record.url?scp=84855285008&partnerID=8YFLogxK
U2 - 10.1007/s00359-011-0686-x
DO - 10.1007/s00359-011-0686-x
M3 - Article
C2 - 21983743
AN - SCOPUS:84855285008
SN - 0340-7594
VL - 198
SP - 79
EP - 83
JO - Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology
JF - Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology
IS - 1
ER -