Labeling enhancement of hydrophilic and hydrophobic molecules in tissue sections

Po Hang Tseng; Shu Jen Chiang; Shean Jen Chen; Chen Yuan Dong

doi:10.1117/12.2582702

Labeling enhancement of hydrophilic and hydrophobic molecules in tissue sections

Po Hang Tseng, Shu Jen Chiang, Shean Jen Chen, Chen Yuan Dong^*

^*Corresponding author for this work

Institute of Imaging and Biomedical Photonics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

In this study, the efficiency of uni- and bi-directional molecular diffusion in porcine liver tissue sections was studied. Tissue sections on the order of a few hundred microns in thickness were used. We found that bi-directional diffusion is more efficient in delivering probe molecules. Therefore, bi-directional labeling is useful in visualizing tissue architecture in three-dimensions.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences XXI
Editors	Ammasi Periasamy, Peter T. So, Karsten Konig
Publisher	SPIE
ISBN (Electronic)	9781510641310
DOIs	https://doi.org/10.1117/12.2582702
State	Published - Mar 2021
Event	Multiphoton Microscopy in the Biomedical Sciences XXI 2021 - Virtual, Online, United States Duration: 6 Mar 2021 → 11 Mar 2021

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11648
ISSN (Print)	1605-7422

Conference

Conference	Multiphoton Microscopy in the Biomedical Sciences XXI 2021
Country/Territory	United States
City	Virtual, Online
Period	6/03/21 → 11/03/21

Keywords

Diffusion
Enhanced labeling
Multiphoton imaging
Tissue sections

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10.1117/12.2582702

Cite this

Tseng, P. H., Chiang, S. J., Chen, S. J., & Dong, C. Y. (2021). Labeling enhancement of hydrophilic and hydrophobic molecules in tissue sections. In A. Periasamy, P. T. So, & K. Konig (Eds.), Multiphoton Microscopy in the Biomedical Sciences XXI Article 116481E (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11648). SPIE. https://doi.org/10.1117/12.2582702

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title = "Labeling enhancement of hydrophilic and hydrophobic molecules in tissue sections",

abstract = "In this study, the efficiency of uni- and bi-directional molecular diffusion in porcine liver tissue sections was studied. Tissue sections on the order of a few hundred microns in thickness were used. We found that bi-directional diffusion is more efficient in delivering probe molecules. Therefore, bi-directional labeling is useful in visualizing tissue architecture in three-dimensions.",

keywords = "Diffusion, Enhanced labeling, Multiphoton imaging, Tissue sections",

author = "Tseng, {Po Hang} and Chiang, {Shu Jen} and Chen, {Shean Jen} and Dong, {Chen Yuan}",

year = "2021",

month = mar,

doi = "10.1117/12.2582702",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Ammasi Periasamy and So, {Peter T.} and Karsten Konig",

booktitle = "Multiphoton Microscopy in the Biomedical Sciences XXI",

address = "United States",

}

Tseng, PH, Chiang, SJ, Chen, SJ & Dong, CY 2021, Labeling enhancement of hydrophilic and hydrophobic molecules in tissue sections. in A Periasamy, PT So & K Konig (eds), Multiphoton Microscopy in the Biomedical Sciences XXI., 116481E, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11648, SPIE, Multiphoton Microscopy in the Biomedical Sciences XXI 2021, Virtual, Online, United States, 6/03/21. https://doi.org/10.1117/12.2582702

Labeling enhancement of hydrophilic and hydrophobic molecules in tissue sections. / Tseng, Po Hang; Chiang, Shu Jen; Chen, Shean Jen et al.
Multiphoton Microscopy in the Biomedical Sciences XXI. ed. / Ammasi Periasamy; Peter T. So; Karsten Konig. SPIE, 2021. 116481E (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11648).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Labeling enhancement of hydrophilic and hydrophobic molecules in tissue sections

AU - Tseng, Po Hang

AU - Chiang, Shu Jen

AU - Chen, Shean Jen

AU - Dong, Chen Yuan

PY - 2021/3

Y1 - 2021/3

N2 - In this study, the efficiency of uni- and bi-directional molecular diffusion in porcine liver tissue sections was studied. Tissue sections on the order of a few hundred microns in thickness were used. We found that bi-directional diffusion is more efficient in delivering probe molecules. Therefore, bi-directional labeling is useful in visualizing tissue architecture in three-dimensions.

AB - In this study, the efficiency of uni- and bi-directional molecular diffusion in porcine liver tissue sections was studied. Tissue sections on the order of a few hundred microns in thickness were used. We found that bi-directional diffusion is more efficient in delivering probe molecules. Therefore, bi-directional labeling is useful in visualizing tissue architecture in three-dimensions.

KW - Diffusion

KW - Enhanced labeling

KW - Multiphoton imaging

KW - Tissue sections

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DO - 10.1117/12.2582702

M3 - Conference contribution

AN - SCOPUS:85107772631

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Multiphoton Microscopy in the Biomedical Sciences XXI

A2 - Periasamy, Ammasi

A2 - So, Peter T.

A2 - Konig, Karsten

PB - SPIE

T2 - Multiphoton Microscopy in the Biomedical Sciences XXI 2021

Y2 - 6 March 2021 through 11 March 2021

ER -

Labeling enhancement of hydrophilic and hydrophobic molecules in tissue sections

Abstract

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Keywords

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