Label free sub-picomole level DNA detection with Ag nanoparticle decorated Au nanotip arrays as surface enhanced Raman spectroscopy platform

Hung Chun Lo; Hsin I. Hsiung; Surojit Chattopadhyay; Hsieh Cheng Han; Chia Fu Chen; Jih-Perng Leu; Kuei Hsien Chen; Li Chyong Chen

doi:10.1016/j.bios.2010.10.022

Label free sub-picomole level DNA detection with Ag nanoparticle decorated Au nanotip arrays as surface enhanced Raman spectroscopy platform

Hung Chun Lo, Hsin I. Hsiung, Surojit Chattopadhyay^*, Hsieh Cheng Han, Chia Fu Chen, Jih-Perng Leu, Kuei Hsien Chen, Li Chyong Chen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Label free optical sensing of adenine and thymine oligonucleotides has been achieved at the sub-picomole level using self assembled silver nanoparticles (AgNPs) decorated gold nanotip (AuNT) arrays. The platform consisting of the AuNTs not only aids in efficient bio-immobilization, but also packs AgNPs in a three dimensional high surface area workspace, assisting in surface enhanced Raman scattering (SERS). The use of sub-10. nm AgNPs with optimum inter-particle distance ensures amplification of the chemically specific Raman signals of the adsorbed adenine, thymine, cytosine and guanine molecules in SERS experiments. High temporal stability of the Raman signals ensured reliable and repeatable DNA detection even after three weeks of ambient desk-top conservation. This facile architecture, being three dimensional and non-lithographic, differs from conventional SERS platforms.

Original language	English
Pages (from-to)	2413-2418
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	26
Issue number	5
DOIs	https://doi.org/10.1016/j.bios.2010.10.022
State	Published - 15 Jan 2011

Keywords

Biosensor
DNA
Nanostructure
Surface enhanced Raman spectroscopy
Surface plasmon

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bios.2010.10.022

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title = "Label free sub-picomole level DNA detection with Ag nanoparticle decorated Au nanotip arrays as surface enhanced Raman spectroscopy platform",

abstract = "Label free optical sensing of adenine and thymine oligonucleotides has been achieved at the sub-picomole level using self assembled silver nanoparticles (AgNPs) decorated gold nanotip (AuNT) arrays. The platform consisting of the AuNTs not only aids in efficient bio-immobilization, but also packs AgNPs in a three dimensional high surface area workspace, assisting in surface enhanced Raman scattering (SERS). The use of sub-10. nm AgNPs with optimum inter-particle distance ensures amplification of the chemically specific Raman signals of the adsorbed adenine, thymine, cytosine and guanine molecules in SERS experiments. High temporal stability of the Raman signals ensured reliable and repeatable DNA detection even after three weeks of ambient desk-top conservation. This facile architecture, being three dimensional and non-lithographic, differs from conventional SERS platforms.",

keywords = "Biosensor, DNA, Nanostructure, Surface enhanced Raman spectroscopy, Surface plasmon",

author = "Lo, {Hung Chun} and Hsiung, {Hsin I.} and Surojit Chattopadhyay and Han, {Hsieh Cheng} and Chen, {Chia Fu} and Jih-Perng Leu and Chen, {Kuei Hsien} and Chen, {Li Chyong}",

year = "2011",

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doi = "10.1016/j.bios.2010.10.022",

language = "English",

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journal = "Biosensors and Bioelectronics",

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T1 - Label free sub-picomole level DNA detection with Ag nanoparticle decorated Au nanotip arrays as surface enhanced Raman spectroscopy platform

AU - Lo, Hung Chun

AU - Hsiung, Hsin I.

AU - Chattopadhyay, Surojit

AU - Han, Hsieh Cheng

AU - Chen, Chia Fu

AU - Leu, Jih-Perng

AU - Chen, Kuei Hsien

AU - Chen, Li Chyong

PY - 2011/1/15

Y1 - 2011/1/15

N2 - Label free optical sensing of adenine and thymine oligonucleotides has been achieved at the sub-picomole level using self assembled silver nanoparticles (AgNPs) decorated gold nanotip (AuNT) arrays. The platform consisting of the AuNTs not only aids in efficient bio-immobilization, but also packs AgNPs in a three dimensional high surface area workspace, assisting in surface enhanced Raman scattering (SERS). The use of sub-10. nm AgNPs with optimum inter-particle distance ensures amplification of the chemically specific Raman signals of the adsorbed adenine, thymine, cytosine and guanine molecules in SERS experiments. High temporal stability of the Raman signals ensured reliable and repeatable DNA detection even after three weeks of ambient desk-top conservation. This facile architecture, being three dimensional and non-lithographic, differs from conventional SERS platforms.

AB - Label free optical sensing of adenine and thymine oligonucleotides has been achieved at the sub-picomole level using self assembled silver nanoparticles (AgNPs) decorated gold nanotip (AuNT) arrays. The platform consisting of the AuNTs not only aids in efficient bio-immobilization, but also packs AgNPs in a three dimensional high surface area workspace, assisting in surface enhanced Raman scattering (SERS). The use of sub-10. nm AgNPs with optimum inter-particle distance ensures amplification of the chemically specific Raman signals of the adsorbed adenine, thymine, cytosine and guanine molecules in SERS experiments. High temporal stability of the Raman signals ensured reliable and repeatable DNA detection even after three weeks of ambient desk-top conservation. This facile architecture, being three dimensional and non-lithographic, differs from conventional SERS platforms.

KW - Biosensor

KW - DNA

KW - Nanostructure

KW - Surface enhanced Raman spectroscopy

KW - Surface plasmon

UR - http://www.scopus.com/inward/record.url?scp=78650594595&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2010.10.022

DO - 10.1016/j.bios.2010.10.022

M3 - Article

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AN - SCOPUS:78650594595

SN - 0956-5663

VL - 26

SP - 2413

EP - 2418

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 5

ER -

Label free sub-picomole level DNA detection with Ag nanoparticle decorated Au nanotip arrays as surface enhanced Raman spectroscopy platform

Abstract

Keywords

UN SDGs

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