Label-free protein sensing by employing blue phase liquid crystal

Mon Juan Lee, Chung Huan Chang, Wei Lee

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Blue phases (BPs) are mesophases existing between the isotropic and chiral nematic phases of liquid crystals (LCs). In recent years, blue phase LCs (BPLCs) have been extensively studied in the field of LC science and display technology. However, the application of BPLCs in biosensing has not been explored. In this study, a BPLC-based biosensing technology was developed for the detection and quantitation of bovine serum albumin (BSA). The sensing platform was constructed by assembling an empty cell with two glass slides coated with homeotropic alignment layers and with immobilized BSA atop. The LC cells were heated to isotropic phase and then allowed to cool down to and maintained at distinct BP temperatures for spectral measurements and texture observations. At BSA concentrations below 10-6 g/ml, we observed that the Bragg reflection wavelength blueshifted with increasing concentration of BSA, suggesting that the BP is a potentially sensitive medium in the detection and quantitation of biomolecules. By using the BPLC at 37 °C and the same polymorphic material in the smectic A phase at 20 °C, two linear correlations were established for logarithmic BSA concentrations ranging from 10-9 to 10-6 g/ml and from 10-6 to 10-3 g/ml. Our results demonstrate the potential of BPLCs in biosensing and quantitative analysis of biomolecules.

Original languageEnglish
Article number#281647
Pages (from-to)1712-1720
Number of pages9
JournalBiomedical Optics Express
Volume8
Issue number3
DOIs
StatePublished - 1 Mar 2017

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