Involvement of microrna-296 in the inhibitory effect of epigallocatechin gallate against the migratory properties of anoikis-resistant nasopharyngeal carcinoma cells

Chien Hung Lin, Hsin Hui Wang, Tsung Hsien Chen, Ming Chang Chiang, Peir Haur Hung*, Yann Jang Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Short noncoding endogenous RNAs, including microRNAs (miRNAs), are associated with the development and metastasis of multiple cancers. Epigallocatechin gallate (EGCG), the most active and abundant polyphenol in green tea, plays a crucial role in the modulation of miRNA expression, which is related to changes in cancer progression. In the present study, we explore whether EGCG exerts its suppressive effects on nasopharyngeal carcinoma (NPC) cells through miRNA regulation. The anoikis-resistant sphere-forming NPC cells grown under anchorage-independent conditions exhibit enhanced migratory properties, which were inhibited by EGCG treatment. The miR-296 level was lower in the anoikis-resistant cells than in the monolayer parental cells; however, miR-296 was significantly upregulated after EGCG treatment. We demonstrate that miR-296 is involved in the inhibitory effects of EGCG on the anoikis-resistant NPC cells through the downregulation of signal transducer and activator of transcription 3 (STAT3) activation. Our study is the first to demonstrate that EGCG inhibited the migratory properties of anoikis-resistant cells by modulating the expression of miRNA in NPC cells. Our results indicate the novel effects of EGCG on miRNA regulation to inhibit an invasive phenotype of NPC as well as the regulatory role of miR-296.

Original languageEnglish
Article number973
JournalCancers
Volume12
Issue number4
DOIs
StatePublished - Apr 2020

Keywords

  • Cancer cell migration
  • Epigallocatechin gallate (EGCG)
  • MicroRNA
  • Nasopharyngeal carcinoma (NPC)

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