Investigation of hepatoprotective activity of induced pluripotent stem cells in the mouse model of liver injury

Shih Hwa Chiou*, Chih Hung Chiang, Ching Chih Chang, Hui Chun Huang, Yi Jen Chen, Ping Hsing Tsai, Shaw Yeu Jeng, Shuen Iu Hung, Jung Hung Hsieh, Hsu Shan Huang, Fa Yauh Lee, Shou Dong Lee

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

To date liver transplantation is the only effective treatment for end-stage liver diseases. Considering the potential of pluripotency and differentiation into tridermal lineages, induced pluripotent stem cells (iPSCs) may serve as an alternative of cell-based therapy. Herein, we investigated the effect of iPSC transplantation on thioacetamide- (TAA-) induced acute/fulminant hepatic failure (AHF) in mice. Firstly, we demonstrated that iPSCs had the capacity to differentiate into hepatocyte-like cells (iPSC-Heps) that expressed various hepatic markers, including albumin, -fetoprotein, and hepatocyte nuclear factor-3, and exhibited biological functions. Intravenous transplantation of iPSCs effectively reduced the hepatic necrotic area, improved liver functions and motor activity, and rescued TAA-treated mice from lethal AHF. 1,1 ′ -dioctadecyl-3,3, 3 ′,3 ′ -tetramethylindocarbocyanine perchlorate cell labeling revealed that iPSCs potentially mobilized to the damaged liver area. Taken together, iPSCs can effectively rescue experimental AHF and represent a potentially favorable cell source of cell-based therapy.

Original languageEnglish
Article number219060
JournalJournal of Biomedicine and Biotechnology
Volume2011
DOIs
StatePublished - 2011

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