Investigation of functionalized biomembranes by ATR - SEIRA spectroscopy with polarized light

Reiner Salzer; Christian Kuhne; Gerald Steiner; Wolfgang B. Fischer

doi:10.1117/12.305369

Investigation of functionalized biomembranes by ATR - SEIRA spectroscopy with polarized light

Reiner Salzer, Christian Kuhne, Gerald Steiner^*, Wolfgang B. Fischer

^*Corresponding author for this work

Institute of Biophotonics

Research output: Contribution to journal › Conference article › peer-review

2 Scopus citations

Abstract

Surface enhanced FTIR spectroscopy (SEIRA) with polarized light can be used to investigate the structure of functionalized membranes. We have used this method to characterize lipid membranes containing integral membrane proteins. The Ge ATR crystal was coated by a 9 nm layer of Ag clusters before adsorbing the membrane. The dipoles of the clusters get excited by the evanescent field. The observed spectra show an enhanced absorption of different functional groups. The enhancement factor depends upon the polarization of the incident light and depends upon the orientation of this groups towards the cluster surface. This allows the detection of the structure and orientation of the membrane fragments at the interface of the clusters.

Original language	English
Pages (from-to)	96-100
Number of pages	5
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3250
DOIs	https://doi.org/10.1117/12.305369
State	Published - 1998
Event	Optical Biopsy II - San Jose, CA, United States Duration: 25 Jan 1998 → 26 Jan 1998

Keywords

Biomembrane
Cluster
FTIR spectroscopy
SEIRA
Surface enhanced spectroscopy

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10.1117/12.305369

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title = "Investigation of functionalized biomembranes by ATR - SEIRA spectroscopy with polarized light",

abstract = "Surface enhanced FTIR spectroscopy (SEIRA) with polarized light can be used to investigate the structure of functionalized membranes. We have used this method to characterize lipid membranes containing integral membrane proteins. The Ge ATR crystal was coated by a 9 nm layer of Ag clusters before adsorbing the membrane. The dipoles of the clusters get excited by the evanescent field. The observed spectra show an enhanced absorption of different functional groups. The enhancement factor depends upon the polarization of the incident light and depends upon the orientation of this groups towards the cluster surface. This allows the detection of the structure and orientation of the membrane fragments at the interface of the clusters.",

keywords = "Biomembrane, Cluster, FTIR spectroscopy, SEIRA, Surface enhanced spectroscopy",

author = "Reiner Salzer and Christian Kuhne and Gerald Steiner and Fischer, {Wolfgang B.}",

year = "1998",

doi = "10.1117/12.305369",

language = "English",

volume = "3250",

pages = "96--100",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Optical Biopsy II ; Conference date: 25-01-1998 Through 26-01-1998",

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TY - JOUR

T1 - Investigation of functionalized biomembranes by ATR - SEIRA spectroscopy with polarized light

AU - Salzer, Reiner

AU - Kuhne, Christian

AU - Steiner, Gerald

AU - Fischer, Wolfgang B.

PY - 1998

Y1 - 1998

N2 - Surface enhanced FTIR spectroscopy (SEIRA) with polarized light can be used to investigate the structure of functionalized membranes. We have used this method to characterize lipid membranes containing integral membrane proteins. The Ge ATR crystal was coated by a 9 nm layer of Ag clusters before adsorbing the membrane. The dipoles of the clusters get excited by the evanescent field. The observed spectra show an enhanced absorption of different functional groups. The enhancement factor depends upon the polarization of the incident light and depends upon the orientation of this groups towards the cluster surface. This allows the detection of the structure and orientation of the membrane fragments at the interface of the clusters.

AB - Surface enhanced FTIR spectroscopy (SEIRA) with polarized light can be used to investigate the structure of functionalized membranes. We have used this method to characterize lipid membranes containing integral membrane proteins. The Ge ATR crystal was coated by a 9 nm layer of Ag clusters before adsorbing the membrane. The dipoles of the clusters get excited by the evanescent field. The observed spectra show an enhanced absorption of different functional groups. The enhancement factor depends upon the polarization of the incident light and depends upon the orientation of this groups towards the cluster surface. This allows the detection of the structure and orientation of the membrane fragments at the interface of the clusters.

KW - Biomembrane

KW - Cluster

KW - FTIR spectroscopy

KW - SEIRA

KW - Surface enhanced spectroscopy

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U2 - 10.1117/12.305369

DO - 10.1117/12.305369

M3 - Conference article

AN - SCOPUS:0032226255

SN - 0277-786X

VL - 3250

SP - 96

EP - 100

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Optical Biopsy II

Y2 - 25 January 1998 through 26 January 1998

ER -

Investigation of functionalized biomembranes by ATR - SEIRA spectroscopy with polarized light

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Keywords

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