Intracellular delivery can be achieved by bombarding cells or tissues with accelerated molecules or bacteria without the need for carrier particles

Wei Nan Lian, Cheng Hung Chang, Yin Jhen Chen, Ro Lan Dao, Yun Cin Luo, Jun Yi Chien, Shie Liang Hsieh, Chi Hung Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

To deliver non-permeable molecules into cells, one can utilize protocols such as microinjection, electroporation, liposome-mediated transfection or virus-mediated transfection. However, each method has its own limitations. Here we have developed a new molecular delivery technique where live cells or tissues are bombarded with highly accelerated molecules directly and without the need to conjugate the molecules onto carrier particles, which is essential in conventional "gene gun" experiments. Gene bombardments can be applied to well-differentiated cells, primary cultured cells/neurons or tissue explants, all of which are notoriously difficult to transfect. Exogenously made proteins and even bacteria can be effectively introduced into cells where they can execute their function or replicate. Our experimental results and physical model support the notion that accelerated chemicals, proteins, or microorganisms carry enough momentum to penetrate the plasma membrane. The bombardment process is associated with a transient (∼ 10 min) increase in cell permeability, but such membrane leakage has a minimal adverse effect on cell survival.

Original languageEnglish
Pages (from-to)53-64
Number of pages12
JournalExperimental Cell Research
Volume313
Issue number1
DOIs
StatePublished - 1 Jan 2007

Keywords

  • Gene gun
  • Living cells
  • Permeability
  • Transfection

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