Intestinal crypt organoid: Isolation of intestinal stem cells, in vitro culture, and optical observation

Yun Chen, Chuan Li, Ya Hui Tsai*, Sheng Hong Tseng

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

The isolation and culture of intestinal stem cells (ISCs) was first demonstrated in the very recent decade with the identification of ISC marker Lgr5. The growth of ISCs into crypt organoids provides an in vitro model for studying the mucosal physiology, intestinal cancer tumorigenesis, and intestinal regeneration. Here, we describe two different isolation protocols and demonstrate a fixation method that aids in the confocal observation of the organoids.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages215-228
Number of pages14
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1576
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Confocal observation
  • Crypt organoid
  • Intestinal stem cell (ISC)
  • Isolation
  • Three-dimensional imaging

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