Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging

Yunzhao Wu; Yuqi Zhou; Chun Jung Huang; Hirofumi Kobayashi; Sheng Yan; Yasuyuki Ozeki; Yingli Wu; Chia Wei Sun; Atsushi Yasumoto; Yutaka Yatomi; Cheng Lei; Keisuke Goda

doi:10.1364/OE.380679

Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging

Yunzhao Wu, Yuqi Zhou, Chun Jung Huang, Hirofumi Kobayashi, Sheng Yan, Yasuyuki Ozeki, Yingli Wu, Chia Wei Sun, Atsushi Yasumoto, Yutaka Yatomi, Cheng Lei^*, Keisuke Goda

^*Corresponding author for this work

Department of Photonics

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Optofluidic time-stretch quantitative phase imaging (OTS-QPI) is a powerful tool as it enables high-throughput (>10,000 cell/s) QPI of single live cells. OTS-QPI is based on decoding temporally stretched spectral interferograms that carry the spatial profiles of cells flowing on a microfluidic chip. However, the utility of OTS-QPI is troubled by difficulties in phase retrieval from the high-frequency region of the temporal interferograms, such as phase-unwrapping errors, high instrumentation cost, and large data volume. To overcome these difficulties, we propose and experimentally demonstrate frequency-shifted OTS-QPI by bringing the phase information to the baseband region. Furthermore, to show its boosted utility, we use it to demonstrate image-based classification of leukemia cells with high accuracy over 96% and evaluation of drug-treated leukemia cells via deep learning.

Original language	English
Pages (from-to)	519-532
Number of pages	14
Journal	Optics Express
Volume	28
Issue number	1
DOIs	https://doi.org/10.1364/OE.380679
State	Published - 6 Jan 2020

Access to Document

10.1364/OE.380679

Cite this

@article{6b4401ca1b9c4904a09596bfa015d3e1,

title = "Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging",

abstract = "Optofluidic time-stretch quantitative phase imaging (OTS-QPI) is a powerful tool as it enables high-throughput (>10,000 cell/s) QPI of single live cells. OTS-QPI is based on decoding temporally stretched spectral interferograms that carry the spatial profiles of cells flowing on a microfluidic chip. However, the utility of OTS-QPI is troubled by difficulties in phase retrieval from the high-frequency region of the temporal interferograms, such as phase-unwrapping errors, high instrumentation cost, and large data volume. To overcome these difficulties, we propose and experimentally demonstrate frequency-shifted OTS-QPI by bringing the phase information to the baseband region. Furthermore, to show its boosted utility, we use it to demonstrate image-based classification of leukemia cells with high accuracy over 96% and evaluation of drug-treated leukemia cells via deep learning.",

author = "Yunzhao Wu and Yuqi Zhou and Huang, {Chun Jung} and Hirofumi Kobayashi and Sheng Yan and Yasuyuki Ozeki and Yingli Wu and Sun, {Chia Wei} and Atsushi Yasumoto and Yutaka Yatomi and Cheng Lei and Keisuke Goda",

note = "Publisher Copyright: {\textcopyright} 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.",

year = "2020",

month = jan,

day = "6",

doi = "10.1364/OE.380679",

language = "English",

volume = "28",

pages = "519--532",

journal = "Optics Express",

issn = "1094-4087",

publisher = "Optica Publishing Group",

number = "1",

}

TY - JOUR

T1 - Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging

AU - Wu, Yunzhao

AU - Zhou, Yuqi

AU - Huang, Chun Jung

AU - Kobayashi, Hirofumi

AU - Yan, Sheng

AU - Ozeki, Yasuyuki

AU - Wu, Yingli

AU - Sun, Chia Wei

AU - Yasumoto, Atsushi

AU - Yatomi, Yutaka

AU - Lei, Cheng

AU - Goda, Keisuke

PY - 2020/1/6

Y1 - 2020/1/6

N2 - Optofluidic time-stretch quantitative phase imaging (OTS-QPI) is a powerful tool as it enables high-throughput (>10,000 cell/s) QPI of single live cells. OTS-QPI is based on decoding temporally stretched spectral interferograms that carry the spatial profiles of cells flowing on a microfluidic chip. However, the utility of OTS-QPI is troubled by difficulties in phase retrieval from the high-frequency region of the temporal interferograms, such as phase-unwrapping errors, high instrumentation cost, and large data volume. To overcome these difficulties, we propose and experimentally demonstrate frequency-shifted OTS-QPI by bringing the phase information to the baseband region. Furthermore, to show its boosted utility, we use it to demonstrate image-based classification of leukemia cells with high accuracy over 96% and evaluation of drug-treated leukemia cells via deep learning.

AB - Optofluidic time-stretch quantitative phase imaging (OTS-QPI) is a powerful tool as it enables high-throughput (>10,000 cell/s) QPI of single live cells. OTS-QPI is based on decoding temporally stretched spectral interferograms that carry the spatial profiles of cells flowing on a microfluidic chip. However, the utility of OTS-QPI is troubled by difficulties in phase retrieval from the high-frequency region of the temporal interferograms, such as phase-unwrapping errors, high instrumentation cost, and large data volume. To overcome these difficulties, we propose and experimentally demonstrate frequency-shifted OTS-QPI by bringing the phase information to the baseband region. Furthermore, to show its boosted utility, we use it to demonstrate image-based classification of leukemia cells with high accuracy over 96% and evaluation of drug-treated leukemia cells via deep learning.

UR - http://www.scopus.com/inward/record.url?scp=85077998147&partnerID=8YFLogxK

U2 - 10.1364/OE.380679

DO - 10.1364/OE.380679

M3 - Article

C2 - 32118978

AN - SCOPUS:85077998147

SN - 1094-4087

VL - 28

SP - 519

EP - 532

JO - Optics Express

JF - Optics Express

IS - 1

ER -

Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging

Abstract

Access to Document

Other files and links

Fingerprint

Cite this