Integration of Ni/NiO nanoparticles and a microfluidic ELISA chip to generate a sensing platform forStreptococcus pneumoniaedetection

Chang Ching Weng, Chien Yu Chao, She Ting Wu, Ping Hsien Tsou, Wei Tin Chen*, Bor Ran Li, Yaw-Kuen Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Enzyme-linked immunosorbent assays (ELISAs) are tests that uses antibody recognition and enzyme catalytic activity to identify a substance, and they have been widely used as a diagnostic tool in the clinic. However, performing an ELISA requires various liquid handling steps and long binding times. To solve this problem, we developed a magnetic microfluidic ELISA system (MMF-ELISA). Integration with nickel magnetic nanoparticles can streamline the ELISA process in a fully automated manner forStreptococcus pneumoniaedetection. First, we synthesized paramagnetic surface-oxidized nickel nanoparticles (Ni/NiO NPs) to carry protein G. Then, we assembled a SUM290 (UlaG)-specific antibody on protein G. Finally, we integrated the NPs on a microfluidics chip forS. pneumoniaedetection. The chip contains three different layers to trap the solutions; the bottom layer SiO2is patterned on hydrophobic polymers and integrated with the middle layer PDMS and the top layer PMMA. With Arduino and motor IC, we developed an automated platform forS. pneumoniaedetection. Microfluidic ELISAs can reduce the manual handling and operation time. Furthermore, the developed system can be extended to multiple areas for ELISA-related assays. This economical, rapid and portable system may become a promising platform for sensingS. pneumoniaein clinical applications.

Original languageEnglish
Pages (from-to)28551-28556
Number of pages6
JournalRSC Advances
Volume11
Issue number46
DOIs
StatePublished - 16 Aug 2021

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