Inexpensive handheld device for the construction of high-density nucleic acid arrays

Michèl Schummer*, W. L. Ng, P. S. Nelson, R. E. Bumgarner, L. Hood

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


We report an easy-to-use, 384-pin hand-held arraying and replicating device (ARD) for constructing high-density replicas of nucleic acids and E. coli transformants. We have modified an existing 384-pin tool to include a novel guide system to ensure vertical pin movement and enhance reproducibility. An asymmetric rectangular multiplexing frame is designed to increase the array density to 1536 dots on a standard microplate-size nylon membrane and to reduce the time and effort involved in producing array replicas. Our initial studies used the ARD to construct 1536-dot arrays of ovarian cDNA clones. We have hybridized these arrays with 38P-labeled probes, which resulted in distinctive signals for either visual interpretation or semiautomatic spot detection and signal integration.

Original languageEnglish
Pages (from-to)1087-1092
Number of pages6
Issue number6
StatePublished - 1997


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