Induction of DNA synthesis or apoptosis in mammalian nuclei by Xenopus egg extracts that fail to support the replication of sperm chromatin

Helen Logothetou-Rella; Wei Hsin Sun; Robert F. Brooks

doi:10.1006/cbir.1999.0488

Induction of DNA synthesis or apoptosis in mammalian nuclei by Xenopus egg extracts that fail to support the replication of sperm chromatin

Helen Logothetou-Rella, Wei Hsin Sun, Robert F. Brooks^*

^*Corresponding author for this work

Department of Life Sciences and Institute of Genome Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Cell-free extracts of Xenopus eggs are widely used to study DNA replication in higher eukaryotes. However, with Xenopus sperm chromatin as template, it is a common experience that the efficiency of replication varies from extract to extract, for reasons that are unclear. Here we show that the majority of extracts unable to support sperm replication are nevertheless able to induce DNA synthesis in intact mammalian nuclei. Those that do not, induce apoptosis. (C) 2000 Academic Press.

Original language	English
Pages (from-to)	129-134
Number of pages	6
Journal	Cell Biology International
Volume	24
Issue number	3
DOIs	https://doi.org/10.1006/cbir.1999.0488
State	Published - 2000

Keywords

Apoptosis
DNA synthesis
Mammalian nuclei
Nuclear assembly
Xenopus egg extracts

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10.1006/cbir.1999.0488

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title = "Induction of DNA synthesis or apoptosis in mammalian nuclei by Xenopus egg extracts that fail to support the replication of sperm chromatin",

abstract = "Cell-free extracts of Xenopus eggs are widely used to study DNA replication in higher eukaryotes. However, with Xenopus sperm chromatin as template, it is a common experience that the efficiency of replication varies from extract to extract, for reasons that are unclear. Here we show that the majority of extracts unable to support sperm replication are nevertheless able to induce DNA synthesis in intact mammalian nuclei. Those that do not, induce apoptosis. (C) 2000 Academic Press.",

keywords = "Apoptosis, DNA synthesis, Mammalian nuclei, Nuclear assembly, Xenopus egg extracts",

author = "Helen Logothetou-Rella and Sun, {Wei Hsin} and Brooks, {Robert F.}",

note = "Funding Information: We thank M. Hola for help in preparing the Xenopus egg extracts used here. This work was supported in part by grants from the Medical Research Council and the Wellcome Trust.",

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doi = "10.1006/cbir.1999.0488",

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journal = "Cell Biology International",

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T1 - Induction of DNA synthesis or apoptosis in mammalian nuclei by Xenopus egg extracts that fail to support the replication of sperm chromatin

AU - Logothetou-Rella, Helen

AU - Sun, Wei Hsin

AU - Brooks, Robert F.

N1 - Funding Information: We thank M. Hola for help in preparing the Xenopus egg extracts used here. This work was supported in part by grants from the Medical Research Council and the Wellcome Trust.

PY - 2000

Y1 - 2000

N2 - Cell-free extracts of Xenopus eggs are widely used to study DNA replication in higher eukaryotes. However, with Xenopus sperm chromatin as template, it is a common experience that the efficiency of replication varies from extract to extract, for reasons that are unclear. Here we show that the majority of extracts unable to support sperm replication are nevertheless able to induce DNA synthesis in intact mammalian nuclei. Those that do not, induce apoptosis. (C) 2000 Academic Press.

AB - Cell-free extracts of Xenopus eggs are widely used to study DNA replication in higher eukaryotes. However, with Xenopus sperm chromatin as template, it is a common experience that the efficiency of replication varies from extract to extract, for reasons that are unclear. Here we show that the majority of extracts unable to support sperm replication are nevertheless able to induce DNA synthesis in intact mammalian nuclei. Those that do not, induce apoptosis. (C) 2000 Academic Press.

KW - Apoptosis

KW - DNA synthesis

KW - Mammalian nuclei

KW - Nuclear assembly

KW - Xenopus egg extracts

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AN - SCOPUS:0033757683

SN - 1065-6995

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EP - 134

JO - Cell Biology International

JF - Cell Biology International

IS - 3

ER -

Induction of DNA synthesis or apoptosis in mammalian nuclei by Xenopus egg extracts that fail to support the replication of sperm chromatin

Abstract

Keywords

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