Induction of apoptotic death of human hepatocellular carcinoma (HepG2) cells by ethanolic extract from litchi (Litchi chinensis Sonn.) flower

Jau Tien Lin, Yuan Yen Chang, Yi Chen Chen, Chao Chin Hu, Yu Pei Chang, Shih Han Hsu, Deng Jye Yang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The cytotoxic activity of litchi (. Litchi chinensis Sonn.) flower ethanolic extract (LFEE) against A549 (human lung adenocarcinoma), KB (human nasopharyngeal carcinoma), MCF-7 (human breast adenocarcinoma) and HepG2 (human hepatocellular carcinoma) cells was evaluated. LFEE exhibited better anti-proliferative action against HepG2 cells, which induced G2/M phase arrest and apoptosis in HepG2 cells. HepG2 cells treated with LFEE (0.3 mg/mL) enhanced expressions of p53, tBid, Bad and Bax, along with decreased expressions of Bcl-2 and Bcl-xL, and induced the release of cytochrome c from mitochondria to cytosol. That also accompanied by activation of caspases-3, -8 and -9 and cleavage of poly (adenosine diphosphate (ADP)-ribose) polymerase (PARP); furthermore, down-regulations of phosphoinositide 3 kinase (PI3K), Akt, Akt phosphorylation and Bad phosphorylation could also be observed. Five flavonoids (total amount, 101.07 mg/g dried extract (g DE)), nine phenolic acids (total amount, 55.07 mg/ g DE) and proanthocyanidin A2 (81.20 mg/ g DE) could be determined in LFEE.

Original languageEnglish
Pages (from-to)100-109
Number of pages10
JournalJournal of Functional Foods
Volume19
DOIs
StatePublished - Dec 2015

Keywords

  • Apoptosis
  • Bcl-2 family proteins
  • Caspase
  • Hep G2 cell
  • Litchi (Litchi chinensis Sonn.) flower
  • PI3K/Akt

Fingerprint

Dive into the research topics of 'Induction of apoptotic death of human hepatocellular carcinoma (HepG2) cells by ethanolic extract from litchi (Litchi chinensis Sonn.) flower'. Together they form a unique fingerprint.

Cite this