In vivo wound healing diagnosis with second harmonic and fluorescence lifetime imaging

Gitanjal Deka, Wei Wen Wu, Fu Jen Kao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Skin wounds heal when a series of cell lineages are triggered, followed by collagen deposition, to reconstruct damaged tissues. This study evaluates the regeneration of collagen and change in cellular metabolic rate in vivo during wound healing in rats, with second harmonic generation (SHG) and fluorescence lifetime imaging microscopy respectively. The metabolic rate of cells is reflected through the lifetime of the autofluorescence from the co-enzyme protein, reduced nicotinamide adenine dinucleotide, due to its change in the relative concentration of bound and free forms. A higher than normal cellular metabolic rate is observed during the first week of healing, which decreases gradually after eight days of wound formation. SHG signal intensity change indicates the net degradation of collagen during the inflammatory phase, and net regeneration begins on day five. Eventually, the quantity of collagen increases gradually to form a scar tissue as the final product. Importantly, this work demonstrates the feasibility of an in vivo imaging approach for a normal wound on rat skin, which has the potential to supplement the noninvasive clinical diagnosis of wounds.

Original languageEnglish
Article number61222
JournalJournal of Biomedical Optics
Volume18
Issue number6
DOIs
StatePublished - 2013

Keywords

  • collagen
  • fluorescence lifetime imaging microscopy
  • reduced nicotinamide adenine dinucleotide
  • second harmonic generation
  • wound healing

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