In vivo blood lactic acid monitoring using microdialysis and surface-enhanced Raman spectroscopy

Po Hsiang Hsu*, Tung Hu Tsai, Huihua Kenny Chiang

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations


Blood lactic acid concentration is an important indicator for physiological functions. To develop a rapid and sensitive measurement technique for monitoring blood lactic acid may provide a useful tool in clinical diagnosis. We proposed to develop a microdialysis surface-enhanced Raman spectroscopy (microdialysis-SERS) approach to filter/reduce interference from other large metabolites in blood and enhance the detection sensitivity for blood lactic acid. In this study, a microdialysis probe was constructed using 13 kDa cut-off dialysis membrane. The dialysate was mixed with 50 nm Ag colloidal nanoparticles automatically in a micro-fluid chamber for SERS detection under blood microdialysis of Sprague-Dawley rat. The linear range of SERS-lactic acid measurement is 10-5∼3x10-4 M with R2 value of 0.99. The optimal mixing flow rate of nanoparticles is 18 μl/min under microdialysis at constant flow rate (2 μl/min). Real time lactic acid monitoring in vivo also has been demonstrated using microdialysis-SERS system.

Original languageEnglish
Title of host publicationNanobiosystems
Subtitle of host publicationProcessing, Characterization, and Applications
StatePublished - 2008
EventNanobiosystems: Processing, Characterization, and Applications - San Diego, CA, United States
Duration: 12 Aug 200814 Aug 2008

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X


ConferenceNanobiosystems: Processing, Characterization, and Applications
Country/TerritoryUnited States
CitySan Diego, CA


  • 13 kDa cut-off dialysis membrane
  • Ag colloidal nanoparticles
  • Lactic acid
  • Microdialysis
  • Microdialysis probe
  • Surface-enhanced Raman spectroscopy


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