In vitro and in vivo imaging of peroxynitrite by a ratiometric boronate-based fluorescent probe

Sathyadevi Palanisamy, Pei Yu Wu, Shou Cheng Wu, Yu Jen Chen, Shey-Cherng Tzou, Chih-Hong Wang, Chiao Yun Chen, Yun-Ming Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

Peroxynitrite (ONOO) is an important species involved in many physiopathological processes. Progresses have been made in developing novel fluorescent probes to detect peroxynitrite with relatively high sensitivity and specificity. Herein, we report the synthesis, characterization and biological applications of a new boronate-based fluorescent probe, 4-MB. The studies showed that 4-MB exhibits a dual ratiometric and calorimetric response toward peroxynitrite due to ONOO-triggered oxidative reaction. A possible mechanism of the oxidation reaction was proposed and the reaction product was isolated and characterized using different spectroscopic methods. We have thoroughly demonstrated the utility of 4-MB for intracellular peroxynitrite imaging. Further, we showed that 4-MB can be potentially employed to visualize exogenous and endogenous peroxynitrite in RAW264.7 macrophages, EAhy926 cells, zebrafish and in live tissues from a high-fat diet-induced obese mouse model.

Original languageEnglish
Pages (from-to)849-856
Number of pages8
JournalBiosensors and Bioelectronics
Volume91
Issue number15
DOIs
StatePublished - 15 May 2017

Keywords

  • Fluorescence
  • Imaging
  • Peroxynitrite
  • Sensor

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