In vitro and in vivo imaging of nitroxyl with copper fluorescent probe in living cells and zebrafish

Sathyadevi Palanisamy, Yu Liang Wang, Yu Jen Chen, Chiao Yun Chen, Fu Te Tsai, Wen Feng Liaw, Yun-Ming Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Nitroxyl (HNO) plays a critical role in many physiological processes which includes vasorelaxation in heart failure, neuroregulation, and myocardial contractility. Powerful imaging tools are required to obtain information for understanding the mechanisms involved in these in vivo processes. In order to develop a rapid and high sensitive probe for HNO detection in living cells and the zebrafish model organism, 2-((2-(benzothiazole-2yl)benzylidene) amino)benzoic acid (AbTCA) as a ligand, and its corresponding copper(II) complex Cu(II)-AbTCA were synthesized. The reaction results of Cu(II)-AbTCA with Angeli's salt showed that Cu(II)-AbTCA could detect HNO quantitatively in a range of 40-360μM with a detection limit of 9.05μM. Furthermore, Cu(II)-AbTCA is more selective towards HNO over other biological species including thiols, reactive nitrogen, and reactive oxygen species. Importantly, Cu(II)-AbTCA was successfully applied to detect HNO in living cells and zebrafish. The collective data reveals that Cu(II)-AbTCA could be used as a potential probe for HNO detection in living systems.

Original languageEnglish
Article number2551
Pages (from-to)1-15
Number of pages15
Issue number10
StatePublished - 6 Oct 2018


  • Fluorescent probe
  • Living system
  • Nitroxyl
  • Zebrafish (danio rerio)


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