In situ patterning and controlling living cells by utilizing femtosecond laser

Kazunori Okano*, Hsin-Yun Hsu, Yaw-Kuen Li, Hiroshi Masuhara

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

19 Scopus citations


Photo-induced processes have high potential in in situ patterning and controlling living cells, whose developments are introduced and recent progresses by utilizing femtosecond laser are described. Photochemical and photothermal surface modification performed by conventional light and nanosecond laser irradiation is summarized and their applicability is considered. Femtosecond laser ablation has superior features due to its photomechanical mechanism, which is confirmed by ultrafast spectroscopy and imaging of a model film under laser ablation. Femtosecond laser ablation of physiological solutions generates shockwave and cavitation bubbles, which is employed for patterning and manipulating living cells. Femtosecond laser ablation fabricating cytophobic and cytophilic domains enable us to form living cell patterns and to study cell migration and cell–cell interaction. Finally summary and perspective are presented.

Original languageEnglish
Pages (from-to)1-28
Number of pages28
JournalJournal of Photochemistry and Photobiology C: Photochemistry Reviews
StatePublished - Sep 2016


  • Cell migration
  • Cell-cell interaction
  • Femtosecond laser ablation
  • Femtosecond laser cavitation
  • Living cell manipulation
  • Living cell patterning


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