IMS method performance analyses for Giardia in water under differing conditions

Bing M. Hsu; Ch-Hpin Huang

doi:10.1007/s10661-006-9462-8

IMS method performance analyses for Giardia in water under differing conditions

Bing M. Hsu^*, Ch-Hpin Huang

^*Corresponding author for this work

Institute of Environmental Engineering

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Giardia under USEPA Method 1623. In this study, IMS was evaluated on the basis of recovery efficiencies for Giardia cysts under various IMS operation conditions. Significant change in recovery was observed by altering the debris ratio of water samples. Notably, cyst recovery efficiencies utilizing IMS dropped with increased turbidity, and results for varying dosages of magnetic beads and cysts indicate that 1/100 immunomagnetic beads is sufficient to conjugate large numbers of cysts. Changing vessel volume and replacing the sample buffer had no significant effect on cyst recovery efficiencies.

Original language	English
Pages (from-to)	129-134
Number of pages	6
Journal	Environmental Monitoring and Assessment
Volume	131
Issue number	1-3
DOIs	https://doi.org/10.1007/s10661-006-9462-8
State	Published - 1 Aug 2007

Keywords

Giardia
Immunomagnetic separation (IMS)
Method1623

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10.1007/s10661-006-9462-8

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abstract = "Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Giardia under USEPA Method 1623. In this study, IMS was evaluated on the basis of recovery efficiencies for Giardia cysts under various IMS operation conditions. Significant change in recovery was observed by altering the debris ratio of water samples. Notably, cyst recovery efficiencies utilizing IMS dropped with increased turbidity, and results for varying dosages of magnetic beads and cysts indicate that 1/100 immunomagnetic beads is sufficient to conjugate large numbers of cysts. Changing vessel volume and replacing the sample buffer had no significant effect on cyst recovery efficiencies.",

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AU - Huang, Ch-Hpin

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N2 - Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Giardia under USEPA Method 1623. In this study, IMS was evaluated on the basis of recovery efficiencies for Giardia cysts under various IMS operation conditions. Significant change in recovery was observed by altering the debris ratio of water samples. Notably, cyst recovery efficiencies utilizing IMS dropped with increased turbidity, and results for varying dosages of magnetic beads and cysts indicate that 1/100 immunomagnetic beads is sufficient to conjugate large numbers of cysts. Changing vessel volume and replacing the sample buffer had no significant effect on cyst recovery efficiencies.

AB - Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Giardia under USEPA Method 1623. In this study, IMS was evaluated on the basis of recovery efficiencies for Giardia cysts under various IMS operation conditions. Significant change in recovery was observed by altering the debris ratio of water samples. Notably, cyst recovery efficiencies utilizing IMS dropped with increased turbidity, and results for varying dosages of magnetic beads and cysts indicate that 1/100 immunomagnetic beads is sufficient to conjugate large numbers of cysts. Changing vessel volume and replacing the sample buffer had no significant effect on cyst recovery efficiencies.

KW - Giardia

KW - Immunomagnetic separation (IMS)

KW - Method1623

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EP - 134

JO - Environmental Monitoring and Assessment

JF - Environmental Monitoring and Assessment

IS - 1-3

ER -

IMS method performance analyses for Giardia in water under differing conditions

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Keywords

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