Impact of apurinic/apyrimidinic endonuclease 1/redox factor-1 on treatment response and survival in oral squamous cell carcinoma

Kan Tai Hsia; Chung Ji Liu; Kwei Mar; Li Han Lin; Chun Shu Lin; Ming Fang Cheng; Herng Sheng Lee; Shang Yi Chiu

doi:10.1002/hed.23927

Impact of apurinic/apyrimidinic endonuclease 1/redox factor-1 on treatment response and survival in oral squamous cell carcinoma

Kan Tai Hsia^*, Chung Ji Liu, Kwei Mar, Li Han Lin, Chun Shu Lin, Ming Fang Cheng, Herng Sheng Lee, Shang Yi Chiu

^*Corresponding author for this work

Institute of Oral Biology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Background Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a multifunctional protein involved in DNA repair and redox signaling. The purpose of this study was to investigate the relationship between APE1/Ref-1 expression and clinicopathological features, survival, and treatment response in patients with oral squamous cell carcinoma (OSCC) and cell lines. Methods APE1/Ref-1 expression in OSCC was evaluated by immunohistochemistry, and its relationship to patient outcomes and treatment response was assessed statistically. The effects of stable short hairpin (sh)RNA-mediated knockdown of APE1/Ref-1 on cell survival, migration, and chemoradiation sensitivity were determined in OSCC cell lines. Results APE1/Ref-1 immunostaining was correlated with positive lymph node status, and higher APE1/Ref-1 expression was significantly associated with poor prognosis and reduced treatment response. Consistent with the clinical studies, APE1/Ref-1 expression in OSCC cell lines was implicated in the regulation of migration and cisplatin-induced apoptosis. Conclusion Elevated APE1/Ref-1 expression may be used to predict poor survival and may confer chemoresistance in OSCC.

Original language	English
Pages (from-to)	550-559
Number of pages	10
Journal	Head and Neck
Volume	38
Issue number	4
DOIs	https://doi.org/10.1002/hed.23927
State	Published - 1 Apr 2016

Keywords

apurinic/apyrimidinic endonuclease/redox factor 1 (APE1/Ref-1)
cisplatin
immunohistochemistry
knockdown
oral squamous cell carcinoma (OSCC)

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@article{a36ff72f23704c559d131c5f16b8ed4f,

title = "Impact of apurinic/apyrimidinic endonuclease 1/redox factor-1 on treatment response and survival in oral squamous cell carcinoma",

abstract = "Background Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a multifunctional protein involved in DNA repair and redox signaling. The purpose of this study was to investigate the relationship between APE1/Ref-1 expression and clinicopathological features, survival, and treatment response in patients with oral squamous cell carcinoma (OSCC) and cell lines. Methods APE1/Ref-1 expression in OSCC was evaluated by immunohistochemistry, and its relationship to patient outcomes and treatment response was assessed statistically. The effects of stable short hairpin (sh)RNA-mediated knockdown of APE1/Ref-1 on cell survival, migration, and chemoradiation sensitivity were determined in OSCC cell lines. Results APE1/Ref-1 immunostaining was correlated with positive lymph node status, and higher APE1/Ref-1 expression was significantly associated with poor prognosis and reduced treatment response. Consistent with the clinical studies, APE1/Ref-1 expression in OSCC cell lines was implicated in the regulation of migration and cisplatin-induced apoptosis. Conclusion Elevated APE1/Ref-1 expression may be used to predict poor survival and may confer chemoresistance in OSCC.",

keywords = "apurinic/apyrimidinic endonuclease/redox factor 1 (APE1/Ref-1), cisplatin, immunohistochemistry, knockdown, oral squamous cell carcinoma (OSCC)",

author = "Hsia, {Kan Tai} and Liu, {Chung Ji} and Kwei Mar and Lin, {Li Han} and Lin, {Chun Shu} and Cheng, {Ming Fang} and Lee, {Herng Sheng} and Chiu, {Shang Yi}",

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year = "2016",

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doi = "10.1002/hed.23927",

language = "English",

volume = "38",

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T1 - Impact of apurinic/apyrimidinic endonuclease 1/redox factor-1 on treatment response and survival in oral squamous cell carcinoma

AU - Hsia, Kan Tai

AU - Liu, Chung Ji

AU - Mar, Kwei

AU - Lin, Li Han

AU - Lin, Chun Shu

AU - Cheng, Ming Fang

AU - Lee, Herng Sheng

AU - Chiu, Shang Yi

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Background Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a multifunctional protein involved in DNA repair and redox signaling. The purpose of this study was to investigate the relationship between APE1/Ref-1 expression and clinicopathological features, survival, and treatment response in patients with oral squamous cell carcinoma (OSCC) and cell lines. Methods APE1/Ref-1 expression in OSCC was evaluated by immunohistochemistry, and its relationship to patient outcomes and treatment response was assessed statistically. The effects of stable short hairpin (sh)RNA-mediated knockdown of APE1/Ref-1 on cell survival, migration, and chemoradiation sensitivity were determined in OSCC cell lines. Results APE1/Ref-1 immunostaining was correlated with positive lymph node status, and higher APE1/Ref-1 expression was significantly associated with poor prognosis and reduced treatment response. Consistent with the clinical studies, APE1/Ref-1 expression in OSCC cell lines was implicated in the regulation of migration and cisplatin-induced apoptosis. Conclusion Elevated APE1/Ref-1 expression may be used to predict poor survival and may confer chemoresistance in OSCC.

AB - Background Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a multifunctional protein involved in DNA repair and redox signaling. The purpose of this study was to investigate the relationship between APE1/Ref-1 expression and clinicopathological features, survival, and treatment response in patients with oral squamous cell carcinoma (OSCC) and cell lines. Methods APE1/Ref-1 expression in OSCC was evaluated by immunohistochemistry, and its relationship to patient outcomes and treatment response was assessed statistically. The effects of stable short hairpin (sh)RNA-mediated knockdown of APE1/Ref-1 on cell survival, migration, and chemoradiation sensitivity were determined in OSCC cell lines. Results APE1/Ref-1 immunostaining was correlated with positive lymph node status, and higher APE1/Ref-1 expression was significantly associated with poor prognosis and reduced treatment response. Consistent with the clinical studies, APE1/Ref-1 expression in OSCC cell lines was implicated in the regulation of migration and cisplatin-induced apoptosis. Conclusion Elevated APE1/Ref-1 expression may be used to predict poor survival and may confer chemoresistance in OSCC.

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KW - cisplatin

KW - immunohistochemistry

KW - knockdown

KW - oral squamous cell carcinoma (OSCC)

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Impact of apurinic/apyrimidinic endonuclease 1/redox factor-1 on treatment response and survival in oral squamous cell carcinoma

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