Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy

Nirmal Mazumder, Jianjun Qiu, Chih Wei Hu, Fu Jen Kao

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

We analyzed the polarization states of second harmonic generation (SHG) signals from starch granules and type I collagen through a four-channel photon counting based Stokes-polarimeter. The 2D SHG images of samples are reconstructed using various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), as well as the anisotropy from the acquired Stokes parameters. Furthermore, we have demonstrated that the polarization parameters are changes at different input polarizations and focusing depths.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XIII
DOIs
StatePublished - 2013
EventMultiphoton Microscopy in the Biomedical Sciences XIII - San Francisco, CA, United States
Duration: 3 Feb 20135 Feb 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8588
ISSN (Print)1605-7422

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XIII
Country/TerritoryUnited States
CitySan Francisco, CA
Period3/02/135/02/13

Keywords

  • And metrology
  • Instrumentation
  • Measurement
  • Nonlinear microscopy
  • Polarimetry
  • Ultrafast optics

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