Imaging dental sections with polarization-resolved SHG and timeresolved autofluorescence

Jun Huang Chen, Po Yen Lin, Stephen C.Y. Hsu, Fu Jen Kao

Research output: Contribution to journalConference articlepeer-review

2 Scopus citations


In this study, we are using two-photon (2-p) excited autofluorescence and second harmonic (SH) as imaging modalities to investigate dental sections that contains the enamel and the dentin. The use of near-infrared wavelengths for multiphoton excitation greatly facilitates the observation of these sections due to the hard tissue's larger index of refraction and highly scattering nature. Clear imaging can be achieved without feature altering preparation procedures of the samples. Specifically, we perform polarization resolving on SH and lifetime analysis on autofluorescence. Polarization resolved SH reflects the preferred orientation of collagen while very different autofluorescence lifetimes are observed from the dentin and the enamel. The origin of 2-p autofluorescence and SH signals are attributed to hydroxyapatite crystals and collagen fibrils, respectively. Hydroxyapatite is found to be present throughout the sections while collagen fibrils exist only in the dentin and dentinoenamel junctions.

Original languageEnglish
Article number71831N
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - 2009
EventMultiphoton Microscopy in the Biomedical Sciences IX - San Jose, CA, United States
Duration: 25 Jan 200927 Jan 2009


  • Anisotropy
  • Autofluorescence
  • Fluorescence lifetime imaging microscopy
  • Second harmonic generation
  • Two-photon excitation


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