IL-7 receptor signaling is necessary for stage transition in adult B cell development through up-regulation of EBF

Kazu Kikuchi, Anne Y. Lai, Chia Lin Hsu, Motonari Kondo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

188 Scopus citations

Abstract

Cytokine receptor signals have been suggested to stimulate cell differentiation during hemato/lymphopoiesis. Such action, however, has not been clearly demonstrated. Here, we show that adult B cell development in IL-7 -/- and IL-7Rα-/- mice is arrested at the pre-pro-B cell stage due to insufficient expression of the B cell-specific transcription factor EBF and its target genes, which form a transcription factor network in determining B lineage specification. EBF expression is restored in IL-7 -/- pre-pro-B cells upon IL-7 stimulation or in IL-7Rα -/- pre-pro-B cells by activation of STAT5, a major signaling molecule downstream of the IL-7R signaling pathway. Furthermore, enforced EBF expression partially rescues B cell development in IL-7Rα-/- mice. Thus, IL-7 receptor signaling is a participant in the formation of the transcription factor network during B lymphopoiesis by up-regulating EBF, allowing stage transition from the pre-pro-B to further maturational stages.

Original languageEnglish
Pages (from-to)1197-1203
Number of pages7
JournalJournal of Experimental Medicine
Volume201
Issue number8
DOIs
StatePublished - 18 Apr 2005

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