Abstract
This protocol demonstrates how to use MACS software to call peaks in a ChIP-seq data set. MACS is one of the most widely used peak-calling programs. The protocol describes how to run MACS in a UNIX environment and on Galaxy and how to extract the sequences of ±50 bp around the summits of the 500 MACS peaks with the most significant P values.
Original language | English |
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Pages (from-to) | 165-172 |
Number of pages | 8 |
Journal | Cold Spring Harbor Protocols |
Volume | 2017 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2017 |