Identification of the high molecular weight isoform of phostensin

Yu Shan Lin, Hsien Lu Huang, Wei Ting Liu, Ta Hsien Lin*, Hsien Bin Huang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Phostensin is encoded by KIAA1949. 5′-RACEanalysis has been used to identify the translation start site of phostensin mRNA, indicating that it encodes 165 amino acids with an apparent molecular weight of 26 kDa on SDS-PAGE. This low-molecular-weight phostensin is present in human peripheral blood mononuclear cells and many leukemic cell lines. Phostensin is a protein phosphatase-1(PP1) binding protein. It also contains one actin-binding motif at its C-terminal region and binds to the pointed ends of actin filaments, modulating actin dynamics. In the current study, a high-molecular-weight phostensin is identified by using immunoprecipitationin combination with a proteomic approach. This new species of phostensin is also encoded by KIAA1949 and consists of 613 amino acids with an apparent molecular weight of 110 kDa on SDS-PAGE. The low-molecular-weight and high-molecular-weight phostensins were named as phostensin-α and phostensin-β, respectively. Although phostensin-α is the C-terminal region of phostensin-β, it is not degraded from phostensin-β. Phostensin-β is capable of associating with PP1 and actin filaments, and is present in many cell lines.

Original languageEnglish
Pages (from-to)1068-1079
Number of pages12
JournalInternational Journal Of Molecular Sciences
Issue number1
StatePublished - 15 Jan 2014


  • Actin-binding protein
  • KIAA1949
  • Phostensin-α
  • Phostensin-β
  • Protein phosphatase-1


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