Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4

Yu Shan Lin; Kuang Yung Huang; Hui Chun Yu; Ming Chi Lu; Cheng Jhong Fan; Hsien Yu Huang Tseng; Bi Yao Jhuang; Su Qin Liu; Ning Sheng Lai; Ta Hsien Lin; Hsien Bin Huang

doi:10.1016/j.bbrc.2020.07.030

Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4

Yu Shan Lin
, Kuang Yung Huang
, Hui Chun Yu
, Ming Chi Lu
, Cheng Jhong Fan
, Hsien Yu Huang Tseng
, Bi Yao Jhuang
, Su Qin Liu
, Ning Sheng Lai
, Ta Hsien Lin
, Hsien Bin Huang^*

^*Corresponding author for this work

Department of Biochemistry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Phostensin (PTS) encoded by KIAA1949 is a protein phosphatase 1 (PP1)-binding protein. In order to explore the cellular functions of PTS, we have searched PTS-binding proteins by using co-immunoprecipitation in combination with shotgun proteomics. Here, we report two novel PTS-binding proteins, Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. PTS associated with EHD proteins was also observed in GST pull-down assays. Immunofluorescence microscopy demonstrated that the complex was co-localized at the endocytic vesicles. EHD proteins have been known to play a critical role in regulation of endocytic transport. Overexpression of PTS-β can attenuate the endocytic trafficking of transferrin.

Original language	English
Pages (from-to)	236-241
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	531
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2020.07.030
State	Published - 15 Oct 2020

Keywords

Dynactin
EHD4
Endocytic recycling
Eps 15 homology domain-containing protein 1 (EHD1)
KIAA1949
Phostensin

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10.1016/j.bbrc.2020.07.030

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Lin, Y. S., Huang, K. Y., Yu, H. C., Lu, M. C., Fan, C. J., Huang Tseng, H. Y., Jhuang, B. Y., Liu, S. Q., Lai, N. S., Lin, T. H., & Huang, H. B. (2020). Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. Biochemical and Biophysical Research Communications, 531(2), 236-241. https://doi.org/10.1016/j.bbrc.2020.07.030

@article{9b4919bcb75f448eb66a71cd0ef69afb,

title = "Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4",

abstract = "Phostensin (PTS) encoded by KIAA1949 is a protein phosphatase 1 (PP1)-binding protein. In order to explore the cellular functions of PTS, we have searched PTS-binding proteins by using co-immunoprecipitation in combination with shotgun proteomics. Here, we report two novel PTS-binding proteins, Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. PTS associated with EHD proteins was also observed in GST pull-down assays. Immunofluorescence microscopy demonstrated that the complex was co-localized at the endocytic vesicles. EHD proteins have been known to play a critical role in regulation of endocytic transport. Overexpression of PTS-β can attenuate the endocytic trafficking of transferrin.",

keywords = "Dynactin, EHD4, Endocytic recycling, Eps 15 homology domain-containing protein 1 (EHD1), KIAA1949, Phostensin",

author = "Lin, \{Yu Shan\} and Huang, \{Kuang Yung\} and Yu, \{Hui Chun\} and Lu, \{Ming Chi\} and Fan, \{Cheng Jhong\} and \{Huang Tseng\}, \{Hsien Yu\} and Jhuang, \{Bi Yao\} and Liu, \{Su Qin\} and Lai, \{Ning Sheng\} and Lin, \{Ta Hsien\} and Huang, \{Hsien Bin\}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

month = oct,

day = "15",

doi = "10.1016/j.bbrc.2020.07.030",

language = "English",

volume = "531",

pages = "236--241",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

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TY - JOUR

T1 - Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4

AU - Lin, Yu Shan

AU - Huang, Kuang Yung

AU - Yu, Hui Chun

AU - Lu, Ming Chi

AU - Fan, Cheng Jhong

AU - Huang Tseng, Hsien Yu

AU - Jhuang, Bi Yao

AU - Liu, Su Qin

AU - Lai, Ning Sheng

AU - Lin, Ta Hsien

AU - Huang, Hsien Bin

PY - 2020/10/15

Y1 - 2020/10/15

N2 - Phostensin (PTS) encoded by KIAA1949 is a protein phosphatase 1 (PP1)-binding protein. In order to explore the cellular functions of PTS, we have searched PTS-binding proteins by using co-immunoprecipitation in combination with shotgun proteomics. Here, we report two novel PTS-binding proteins, Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. PTS associated with EHD proteins was also observed in GST pull-down assays. Immunofluorescence microscopy demonstrated that the complex was co-localized at the endocytic vesicles. EHD proteins have been known to play a critical role in regulation of endocytic transport. Overexpression of PTS-β can attenuate the endocytic trafficking of transferrin.

AB - Phostensin (PTS) encoded by KIAA1949 is a protein phosphatase 1 (PP1)-binding protein. In order to explore the cellular functions of PTS, we have searched PTS-binding proteins by using co-immunoprecipitation in combination with shotgun proteomics. Here, we report two novel PTS-binding proteins, Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. PTS associated with EHD proteins was also observed in GST pull-down assays. Immunofluorescence microscopy demonstrated that the complex was co-localized at the endocytic vesicles. EHD proteins have been known to play a critical role in regulation of endocytic transport. Overexpression of PTS-β can attenuate the endocytic trafficking of transferrin.

KW - Dynactin

KW - EHD4

KW - Endocytic recycling

KW - Eps 15 homology domain-containing protein 1 (EHD1)

KW - KIAA1949

KW - Phostensin

UR - https://www.scopus.com/pages/publications/85089356444

U2 - 10.1016/j.bbrc.2020.07.030

DO - 10.1016/j.bbrc.2020.07.030

M3 - Article

C2 - 32800345

AN - SCOPUS:85089356444

SN - 0006-291X

VL - 531

SP - 236

EP - 241

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4

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