Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4

Yu Shan Lin, Kuang Yung Huang, Hui Chun Yu, Ming Chi Lu, Cheng Jhong Fan, Hsien Yu Huang Tseng, Bi Yao Jhuang, Su Qin Liu, Ning Sheng Lai, Ta Hsien Lin, Hsien Bin Huang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Phostensin (PTS) encoded by KIAA1949 is a protein phosphatase 1 (PP1)-binding protein. In order to explore the cellular functions of PTS, we have searched PTS-binding proteins by using co-immunoprecipitation in combination with shotgun proteomics. Here, we report two novel PTS-binding proteins, Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. PTS associated with EHD proteins was also observed in GST pull-down assays. Immunofluorescence microscopy demonstrated that the complex was co-localized at the endocytic vesicles. EHD proteins have been known to play a critical role in regulation of endocytic transport. Overexpression of PTS-β can attenuate the endocytic trafficking of transferrin.

Original languageEnglish
Pages (from-to)236-241
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume531
Issue number2
DOIs
StatePublished - 15 Oct 2020

Keywords

  • Dynactin
  • EHD4
  • Endocytic recycling
  • Eps 15 homology domain-containing protein 1 (EHD1)
  • KIAA1949
  • Phostensin

Fingerprint

Dive into the research topics of 'Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4'. Together they form a unique fingerprint.

Cite this