Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit

Shu Chen Kao, Chun Yu Chen, Shiu Lan Wang, Jaw Ji Yang, Wei Chuan Hung, Yi Chen Chen, Ning Sheng Lai, Hsin Tzu Liu, Hsien lu Huang, Hong Chen Chen, Ta Hsien Lin*, Hsien bin Huang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

We have identified a novel protein, protein phosphatase 1 F-actin cytoskeleton targeting subunit (phostensin). This protein is encoded by KIAA1949 and was found to associate with protein phosphatase 1 (PP1) in the yeast two-hybrid assay, co-immunoprecipitation, and GST pull-down assay. Northern blot analysis revealed that phostensin mRNA was predominantly distributed in leukocytes and spleen, and phostensin protein was present in crude extracts of human peripheral leukocytes. Immunofluorescence microscopic analysis revealed that the phostensin/PP1 complex was conspicuously localized with the actin cytoskeleton at the cell periphery in Madin-Darby canine kidney (MDCK) epithelial cells. Taken together, our data shows that phostensin targets PP1 to F-actin cytoskeleton. The phostensin/PP1 complex may play a vital role in modulation of actin rearrangements.

Original languageEnglish
Pages (from-to)594-598
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume356
Issue number3
DOIs
StatePublished - 11 May 2007

Keywords

  • Actin cytoskeleton
  • KIAA1949
  • Phostensin
  • Protein phosphatase 1

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