Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit

Shu Chen Kao; Chun Yu Chen; Shiu Lan Wang; Jaw Ji Yang; Wei Chuan Hung; Yi Chen Chen; Ning Sheng Lai; Hsin Tzu Liu; Hsien lu Huang; Hong Chen Chen; Ta Hsien Lin; Hsien bin Huang

doi:10.1016/j.bbrc.2007.03.026

Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit

Shu Chen Kao, Chun Yu Chen, Shiu Lan Wang, Jaw Ji Yang, Wei Chuan Hung, Yi Chen Chen, Ning Sheng Lai, Hsin Tzu Liu, Hsien lu Huang, Hong Chen Chen, Ta Hsien Lin^*, Hsien bin Huang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

We have identified a novel protein, protein phosphatase 1 F-actin cytoskeleton targeting subunit (phostensin). This protein is encoded by KIAA1949 and was found to associate with protein phosphatase 1 (PP1) in the yeast two-hybrid assay, co-immunoprecipitation, and GST pull-down assay. Northern blot analysis revealed that phostensin mRNA was predominantly distributed in leukocytes and spleen, and phostensin protein was present in crude extracts of human peripheral leukocytes. Immunofluorescence microscopic analysis revealed that the phostensin/PP1 complex was conspicuously localized with the actin cytoskeleton at the cell periphery in Madin-Darby canine kidney (MDCK) epithelial cells. Taken together, our data shows that phostensin targets PP1 to F-actin cytoskeleton. The phostensin/PP1 complex may play a vital role in modulation of actin rearrangements.

Original language	English
Pages (from-to)	594-598
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	356
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2007.03.026
State	Published - 11 May 2007

Keywords

Actin cytoskeleton
KIAA1949
Phostensin
Protein phosphatase 1

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10.1016/j.bbrc.2007.03.026

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title = "Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit",

abstract = "We have identified a novel protein, protein phosphatase 1 F-actin cytoskeleton targeting subunit (phostensin). This protein is encoded by KIAA1949 and was found to associate with protein phosphatase 1 (PP1) in the yeast two-hybrid assay, co-immunoprecipitation, and GST pull-down assay. Northern blot analysis revealed that phostensin mRNA was predominantly distributed in leukocytes and spleen, and phostensin protein was present in crude extracts of human peripheral leukocytes. Immunofluorescence microscopic analysis revealed that the phostensin/PP1 complex was conspicuously localized with the actin cytoskeleton at the cell periphery in Madin-Darby canine kidney (MDCK) epithelial cells. Taken together, our data shows that phostensin targets PP1 to F-actin cytoskeleton. The phostensin/PP1 complex may play a vital role in modulation of actin rearrangements.",

keywords = "Actin cytoskeleton, KIAA1949, Phostensin, Protein phosphatase 1",

author = "Kao, {Shu Chen} and Chen, {Chun Yu} and Wang, {Shiu Lan} and Yang, {Jaw Ji} and Hung, {Wei Chuan} and Chen, {Yi Chen} and Lai, {Ning Sheng} and Liu, {Hsin Tzu} and Huang, {Hsien lu} and Chen, {Hong Chen} and Lin, {Ta Hsien} and Huang, {Hsien bin}",

note = "Funding Information: This work was support by grants from the National Science Council, ROC (NSC 95-2320-B-194-005, NSC 94-2320-B-194-005 and NSC 92-2311-B-010-003) and the Taipei Veterans General Hospital, Taiwan, ROC (VGH 92-375, VGH 93-359 and VGH 94-370).",

year = "2007",

month = may,

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doi = "10.1016/j.bbrc.2007.03.026",

language = "English",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit

AU - Kao, Shu Chen

AU - Chen, Chun Yu

AU - Wang, Shiu Lan

AU - Yang, Jaw Ji

AU - Hung, Wei Chuan

AU - Chen, Yi Chen

AU - Lai, Ning Sheng

AU - Liu, Hsin Tzu

AU - Huang, Hsien lu

AU - Chen, Hong Chen

AU - Lin, Ta Hsien

AU - Huang, Hsien bin

N1 - Funding Information: This work was support by grants from the National Science Council, ROC (NSC 95-2320-B-194-005, NSC 94-2320-B-194-005 and NSC 92-2311-B-010-003) and the Taipei Veterans General Hospital, Taiwan, ROC (VGH 92-375, VGH 93-359 and VGH 94-370).

PY - 2007/5/11

Y1 - 2007/5/11

N2 - We have identified a novel protein, protein phosphatase 1 F-actin cytoskeleton targeting subunit (phostensin). This protein is encoded by KIAA1949 and was found to associate with protein phosphatase 1 (PP1) in the yeast two-hybrid assay, co-immunoprecipitation, and GST pull-down assay. Northern blot analysis revealed that phostensin mRNA was predominantly distributed in leukocytes and spleen, and phostensin protein was present in crude extracts of human peripheral leukocytes. Immunofluorescence microscopic analysis revealed that the phostensin/PP1 complex was conspicuously localized with the actin cytoskeleton at the cell periphery in Madin-Darby canine kidney (MDCK) epithelial cells. Taken together, our data shows that phostensin targets PP1 to F-actin cytoskeleton. The phostensin/PP1 complex may play a vital role in modulation of actin rearrangements.

AB - We have identified a novel protein, protein phosphatase 1 F-actin cytoskeleton targeting subunit (phostensin). This protein is encoded by KIAA1949 and was found to associate with protein phosphatase 1 (PP1) in the yeast two-hybrid assay, co-immunoprecipitation, and GST pull-down assay. Northern blot analysis revealed that phostensin mRNA was predominantly distributed in leukocytes and spleen, and phostensin protein was present in crude extracts of human peripheral leukocytes. Immunofluorescence microscopic analysis revealed that the phostensin/PP1 complex was conspicuously localized with the actin cytoskeleton at the cell periphery in Madin-Darby canine kidney (MDCK) epithelial cells. Taken together, our data shows that phostensin targets PP1 to F-actin cytoskeleton. The phostensin/PP1 complex may play a vital role in modulation of actin rearrangements.

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SN - 0006-291X

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EP - 598

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit

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Keywords

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