Identification of novel viral interleukin-10 isoforms of human cytomegalovirus AD169

Yi Ling Lin; Pei Ching Chang; Yixiang Wang; Mengtao Li

doi:10.1016/j.virusres.2007.09.011

Identification of novel viral interleukin-10 isoforms of human cytomegalovirus AD169

Yi Ling Lin, Pei Ching Chang, Yixiang Wang, Mengtao Li^*

^*Corresponding author for this work

Institute of Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Two products of human cytomegalovirus (HCMV) UL111a gene have been previously identified to resemble human IL-10 (hIL-10). These viral IL-10s (vIL-10s) are able to induce signal transduction events and biological activities in a variety of cells. In this study, five novel vIL-10 transcripts were identified from HCMV AD169 infected MRC-5 cells. Some vIL-10 isoforms were post-translationally glycosylated, depending on the existence of a predicted N-linked glycosylation site. Similar to hIL-10, four of the vIL-10 isoforms apparently formed putative dimers. Among the different vIL-10 isoforms, vIL-10A significantly induced the phosphorylation of transcription factor STAT3 in THP-1 cells. All identified vIL-10 isoforms were able to form complexes with hIL-10, and enhanced hIL-10-induced STAT3 phosphorylation in different degrees. Identification of diverse forms of vIL-10 suggests that HCMV has developed a sophisticated mechanism to interfere with hIL-10 signaling pathway.

Original language	English
Pages (from-to)	213-223
Number of pages	11
Journal	Virus Research
Volume	131
Issue number	2
DOIs	https://doi.org/10.1016/j.virusres.2007.09.011
State	Published - Feb 2008

Keywords

Cytomegalovirus
IL-10
vIL-10

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virusres.2007.09.011

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title = "Identification of novel viral interleukin-10 isoforms of human cytomegalovirus AD169",

abstract = "Two products of human cytomegalovirus (HCMV) UL111a gene have been previously identified to resemble human IL-10 (hIL-10). These viral IL-10s (vIL-10s) are able to induce signal transduction events and biological activities in a variety of cells. In this study, five novel vIL-10 transcripts were identified from HCMV AD169 infected MRC-5 cells. Some vIL-10 isoforms were post-translationally glycosylated, depending on the existence of a predicted N-linked glycosylation site. Similar to hIL-10, four of the vIL-10 isoforms apparently formed putative dimers. Among the different vIL-10 isoforms, vIL-10A significantly induced the phosphorylation of transcription factor STAT3 in THP-1 cells. All identified vIL-10 isoforms were able to form complexes with hIL-10, and enhanced hIL-10-induced STAT3 phosphorylation in different degrees. Identification of diverse forms of vIL-10 suggests that HCMV has developed a sophisticated mechanism to interfere with hIL-10 signaling pathway.",

keywords = "Cytomegalovirus, IL-10, vIL-10",

author = "Lin, {Yi Ling} and Chang, {Pei Ching} and Yixiang Wang and Mengtao Li",

note = "Funding Information: We thank Drs. Craig S. Miller and Robert J. Jacob for critically reading the manuscript and for helpful discussions. We also thank Jingchun Chen and Dr. Bo Yuan at the Ohio State University for the discussion of sequence comparison of vIL-10 isoforms. This project described was supported by Grant RFA-RR-03-014 from the National Center for Research Resources (NCRR), a component of National Institutes of Health (NIH), and its contents are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH.",

year = "2008",

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doi = "10.1016/j.virusres.2007.09.011",

language = "English",

volume = "131",

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T1 - Identification of novel viral interleukin-10 isoforms of human cytomegalovirus AD169

AU - Lin, Yi Ling

AU - Chang, Pei Ching

AU - Wang, Yixiang

AU - Li, Mengtao

N1 - Funding Information: We thank Drs. Craig S. Miller and Robert J. Jacob for critically reading the manuscript and for helpful discussions. We also thank Jingchun Chen and Dr. Bo Yuan at the Ohio State University for the discussion of sequence comparison of vIL-10 isoforms. This project described was supported by Grant RFA-RR-03-014 from the National Center for Research Resources (NCRR), a component of National Institutes of Health (NIH), and its contents are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH.

PY - 2008/2

Y1 - 2008/2

N2 - Two products of human cytomegalovirus (HCMV) UL111a gene have been previously identified to resemble human IL-10 (hIL-10). These viral IL-10s (vIL-10s) are able to induce signal transduction events and biological activities in a variety of cells. In this study, five novel vIL-10 transcripts were identified from HCMV AD169 infected MRC-5 cells. Some vIL-10 isoforms were post-translationally glycosylated, depending on the existence of a predicted N-linked glycosylation site. Similar to hIL-10, four of the vIL-10 isoforms apparently formed putative dimers. Among the different vIL-10 isoforms, vIL-10A significantly induced the phosphorylation of transcription factor STAT3 in THP-1 cells. All identified vIL-10 isoforms were able to form complexes with hIL-10, and enhanced hIL-10-induced STAT3 phosphorylation in different degrees. Identification of diverse forms of vIL-10 suggests that HCMV has developed a sophisticated mechanism to interfere with hIL-10 signaling pathway.

AB - Two products of human cytomegalovirus (HCMV) UL111a gene have been previously identified to resemble human IL-10 (hIL-10). These viral IL-10s (vIL-10s) are able to induce signal transduction events and biological activities in a variety of cells. In this study, five novel vIL-10 transcripts were identified from HCMV AD169 infected MRC-5 cells. Some vIL-10 isoforms were post-translationally glycosylated, depending on the existence of a predicted N-linked glycosylation site. Similar to hIL-10, four of the vIL-10 isoforms apparently formed putative dimers. Among the different vIL-10 isoforms, vIL-10A significantly induced the phosphorylation of transcription factor STAT3 in THP-1 cells. All identified vIL-10 isoforms were able to form complexes with hIL-10, and enhanced hIL-10-induced STAT3 phosphorylation in different degrees. Identification of diverse forms of vIL-10 suggests that HCMV has developed a sophisticated mechanism to interfere with hIL-10 signaling pathway.

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Identification of novel viral interleukin-10 isoforms of human cytomegalovirus AD169

Abstract

Keywords

UN SDGs

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