Highly conserved influenza A virus epitope sequences as candidates of H3N2 flu vaccine targets

Ko Wen Wu; Chih Yi Chien; Shiao Wen Li; Chwan Chuen King; Chuan Hsiung Chang

doi:10.1016/j.ygeno.2012.06.003

Highly conserved influenza A virus epitope sequences as candidates of H3N2 flu vaccine targets

Ko Wen Wu, Chih Yi Chien, Shiao Wen Li, Chwan Chuen King, Chuan Hsiung Chang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

This study focused on identifying the conserved epitopes in a single subtype A (H3N2)-as candidates for vaccine targets. We identified a total of 32 conserved epitopes in four viral proteins [22 HA, 4PB1, 3 NA, 3 NP]. Evaluation of conserved epitopes in coverage during 1968-2010 revealed that (1) 12 HA conserved epitopes were highly present in the circulating viruses; (2) the remaining 10 HA conserved epitopes appeared with lower percentage but a significantly increasing trend after 1989 [p < 0.001]; and (3) the conserved epitopes in NA, NP and PB1 are also highly frequent in wild-type viruses. These conserved epitopes also covered an extremely high percentage of the 16 vaccine strains during the 42. year period. The identification of highly conserved epitopes using our approach can also be applied to develop broad-spectrum vaccines.

Original language	English
Pages (from-to)	102-109
Number of pages	8
Journal	Genomics
Volume	100
Issue number	2
DOIs	https://doi.org/10.1016/j.ygeno.2012.06.003
State	Published - Aug 2012

Keywords

Bioinformatics
Broad-spectrum vaccine
Epitope
Influenza virus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ygeno.2012.06.003

Cite this

@article{80626dbab0cb40babac089b11bcd4f41,

title = "Highly conserved influenza A virus epitope sequences as candidates of H3N2 flu vaccine targets",

abstract = "This study focused on identifying the conserved epitopes in a single subtype A (H3N2)-as candidates for vaccine targets. We identified a total of 32 conserved epitopes in four viral proteins [22 HA, 4PB1, 3 NA, 3 NP]. Evaluation of conserved epitopes in coverage during 1968-2010 revealed that (1) 12 HA conserved epitopes were highly present in the circulating viruses; (2) the remaining 10 HA conserved epitopes appeared with lower percentage but a significantly increasing trend after 1989 [p < 0.001]; and (3) the conserved epitopes in NA, NP and PB1 are also highly frequent in wild-type viruses. These conserved epitopes also covered an extremely high percentage of the 16 vaccine strains during the 42. year period. The identification of highly conserved epitopes using our approach can also be applied to develop broad-spectrum vaccines.",

keywords = "Bioinformatics, Broad-spectrum vaccine, Epitope, Influenza virus",

author = "Wu, {Ko Wen} and Chien, {Chih Yi} and Li, {Shiao Wen} and King, {Chwan Chuen} and Chang, {Chuan Hsiung}",

note = "Funding Information: The authors thank Dr. Chuan-Liang Kao (National Taiwan University), Dr. Yi-Ming Arthur Chen (National Yang-Ming University) and Dr. Yu-Jiun Chan (Taipei Veterans General Hospital) for providing constructive comments and suggestions that improved this paper. This study was supported by the National Science Council, Taiwan ( NSC 100-2319-B-010-002 ) and the Ministry of Education, Taiwan , Aim for the Top University Plan. ",

year = "2012",

month = aug,

doi = "10.1016/j.ygeno.2012.06.003",

language = "English",

volume = "100",

pages = "102--109",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Highly conserved influenza A virus epitope sequences as candidates of H3N2 flu vaccine targets

AU - Wu, Ko Wen

AU - Chien, Chih Yi

AU - Li, Shiao Wen

AU - King, Chwan Chuen

AU - Chang, Chuan Hsiung

N1 - Funding Information: The authors thank Dr. Chuan-Liang Kao (National Taiwan University), Dr. Yi-Ming Arthur Chen (National Yang-Ming University) and Dr. Yu-Jiun Chan (Taipei Veterans General Hospital) for providing constructive comments and suggestions that improved this paper. This study was supported by the National Science Council, Taiwan ( NSC 100-2319-B-010-002 ) and the Ministry of Education, Taiwan , Aim for the Top University Plan.

PY - 2012/8

Y1 - 2012/8

N2 - This study focused on identifying the conserved epitopes in a single subtype A (H3N2)-as candidates for vaccine targets. We identified a total of 32 conserved epitopes in four viral proteins [22 HA, 4PB1, 3 NA, 3 NP]. Evaluation of conserved epitopes in coverage during 1968-2010 revealed that (1) 12 HA conserved epitopes were highly present in the circulating viruses; (2) the remaining 10 HA conserved epitopes appeared with lower percentage but a significantly increasing trend after 1989 [p < 0.001]; and (3) the conserved epitopes in NA, NP and PB1 are also highly frequent in wild-type viruses. These conserved epitopes also covered an extremely high percentage of the 16 vaccine strains during the 42. year period. The identification of highly conserved epitopes using our approach can also be applied to develop broad-spectrum vaccines.

AB - This study focused on identifying the conserved epitopes in a single subtype A (H3N2)-as candidates for vaccine targets. We identified a total of 32 conserved epitopes in four viral proteins [22 HA, 4PB1, 3 NA, 3 NP]. Evaluation of conserved epitopes in coverage during 1968-2010 revealed that (1) 12 HA conserved epitopes were highly present in the circulating viruses; (2) the remaining 10 HA conserved epitopes appeared with lower percentage but a significantly increasing trend after 1989 [p < 0.001]; and (3) the conserved epitopes in NA, NP and PB1 are also highly frequent in wild-type viruses. These conserved epitopes also covered an extremely high percentage of the 16 vaccine strains during the 42. year period. The identification of highly conserved epitopes using our approach can also be applied to develop broad-spectrum vaccines.

KW - Bioinformatics

KW - Broad-spectrum vaccine

KW - Epitope

KW - Influenza virus

UR - http://www.scopus.com/inward/record.url?scp=84864071078&partnerID=8YFLogxK

U2 - 10.1016/j.ygeno.2012.06.003

DO - 10.1016/j.ygeno.2012.06.003

M3 - Article

C2 - 22698979

AN - SCOPUS:84864071078

SN - 0888-7543

VL - 100

SP - 102

EP - 109

JO - Genomics

JF - Genomics

IS - 2

ER -

Highly conserved influenza A virus epitope sequences as candidates of H3N2 flu vaccine targets

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this