High-throughput plasmid mini preparations facilitated by micro-mixing

Wai Lap Ng*, Michél Schummer, Frank D. Cirisano, Rae Lynn Baldwin, Beth Y. Karlan, Leroy Hood

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


We have developed a reliable high-throughput plasmid isolation system using a 96-well plate format. This system combines a novel glass bead micro-mixing method with modified alkaline lysis and Sephacryl S-500 DNA purification procedures. Mechanical forces generated by vortexing glass beads inside each well of the 96-well plates ensure that the bacterial pellets are homogeneously resuspended, the cells are completely lyzed, and the resulting bacterial lysates are thoroughly mixed with the potassium acetate solution. The vortexing speed and duration for glass bead mixing have been standardized to facilitate plasmid DNA yields without significant adjustments.

Original languageEnglish
Pages (from-to)5045-5047
Number of pages3
JournalNucleic acids research
Issue number24
StatePublished - 15 Dec 1996


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