High-resolution cell manipulation for longstanding load on red blood cells

Chia-Hung Tsai, Mitsuhiro Horade, Hiroaki Ito, Makoto Kaneko, Motomu Tanaka

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

A high-resolution cell manipulation system is presented for investigating red blood cell deformation under long-standing load in this paper. Because the low Reynolds number in microfluidic system, cell position can be manipulated by controlling the flow in a microchannel. A high-speed vision system is embedded in the system for providing cell present position as the feedback signal for the controller while a syringe pump actuated by a piezoelectric actuator is employed for flow control in the channel. The system is utilized for applying longstanding load on human red blood cells. The longstanding load is generated by manipulating a cell into a constriction channel where the cross-sectional size is smaller than the size of the cell. The cell has to deform due to the geometrical constraints of the constriction. Both the system performance and cell response to longstanding load have been evaluated. The manipulation system successfully achieves cell positioning as accurate as 0.24 μm while red blood cells are found always exponentially shrink with respect to time, and an average shrinkage of 1.82μm in 5 minutes is observed. Details of system construction and discussion on the cell response are presented.

Original languageEnglish
Title of host publication2016 IEEE International Conference on Mechatronics and Automation, IEEE ICMA 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages914-919
Number of pages6
ISBN (Electronic)9781509023943
DOIs
StatePublished - 1 Sep 2016
Event13th IEEE International Conference on Mechatronics and Automation, IEEE ICMA 2016 - Harbin, Heilongjiang, China
Duration: 7 Aug 201610 Aug 2016

Publication series

Name2016 IEEE International Conference on Mechatronics and Automation, IEEE ICMA 2016

Conference

Conference13th IEEE International Conference on Mechatronics and Automation, IEEE ICMA 2016
Country/TerritoryChina
CityHarbin, Heilongjiang
Period7/08/1610/08/16

Keywords

  • longstanding load
  • microfluidics
  • red blood cell

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