Harmonic generation microscopy of dental sections

Fu Jen Kao*, Chin Ying Stephen Hsu

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

2 Scopus citations


Ultrafast lasers have found increasing use in scanning optical microscopy due to their very high peak power in generating multiphoton excitations. A mode-locked Ti:sapphire laser is often employed for such purposes. Together with a synchronously pumped optical parametric oscillator (OPO), the spectral range available can be extended to 1050-1300 nm. This broader range available greatly facilitates the excitation of second harmonic generation (SHG) and third harmonic generation (THG) due to better satisfaction of phase matching condition that is achieved with a longer excitation wavelength. Dental sections are then investigated with the contrasts from harmonic generation.

Original languageEnglish
Article number74
Pages (from-to)344-350
Number of pages7
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5630 I
StatePublished - 2005
EventOptics in Health Care and Biomedical Optics: Diagnostics and Treatment II - Beijing, China
Duration: 8 Nov 200412 Nov 2004


  • Laser scanning microscopy
  • Optical parametric oscillator
  • Second harmonic generation
  • Third harmonic generation
  • Two-photon excitation


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