TY - JOUR
T1 - Genome sequence of Halobacterium species NRC-1
AU - Ng, Wailap Victor
AU - Kennedy, Sean P.
AU - Mahairas, Gregory G.
AU - Berquist, Brian
AU - Pan, Min
AU - Shukla, Hem Dutt
AU - Lasky, Stephen R.
AU - Baliga, Nitin S.
AU - Thorsson, Vesteinn
AU - Sbrogna, Jennifer
AU - Swartzell, Steven
AU - Weir, Douglas
AU - Hall, John
AU - Dahl, Timothy A.
AU - Welti, Russell
AU - Goo, Young Ah
AU - Leithauser, Brent
AU - Keller, Kim
AU - Cruz, Randy
AU - Danson, Michael J.
AU - Hough, David W.
AU - Maddocks, Deborah G.
AU - Jablonski, Peter E.
AU - Krebs, Mark P.
AU - Angevine, Christine M.
AU - Dale, Heather
AU - Isenbarger, Thomas A.
AU - Peck, Ronald F.
AU - Pohlschroder, Mechthild
AU - Spudich, John L.
AU - Jung, Kwang Hwan
AU - Alam, Maqsudul
AU - Freitas, Tracey
AU - Hou, Shaobin
AU - Daniels, Charles J.
AU - Dennis, Patrick P.
AU - Omer, Arina D.
AU - Ebhardt, Holger
AU - Lowe, Todd M.
AU - Liang, Ping
AU - Riley, Monica
AU - Hood, Leroy
AU - DasSarma, Shiladitya
PY - 2000/10/24
Y1 - 2000/10/24
N2 - We report the complete sequence of an extreme halophile, Halobacterium sp. NRC-1, harboring a dynamic 2,571,010-bp genome containing 91 insertion sequences representing 12 families and organized into a large chromosome and 2 related minichromosomes. The Halobacterium NRC-1 genome codes for 2,630 predicted proteins, 36% of which are unrelated to any previously reported. Analysis of the genome sequence shows the presence of pathways for uptake and utilization of amino acids, active sodium-proton antiporter and potassium uptake systems, sophisticated photosensory and signal transduction pathways, and DNA replication, transcription, and translation systems resembling more complex eukaryotic organisms. Whole proteome comparisons show the definite archaeal nature of this halophile with additional similarities to the Gram-positive Bacillus subtilis and other bacteria. The ease of culturing Halobacterium and the availability of methods for its genetic manipulation in the laboratory, including construction of gene knockouts and replacements, indicate this halophile can serve as an excellent model system among the archaea.
AB - We report the complete sequence of an extreme halophile, Halobacterium sp. NRC-1, harboring a dynamic 2,571,010-bp genome containing 91 insertion sequences representing 12 families and organized into a large chromosome and 2 related minichromosomes. The Halobacterium NRC-1 genome codes for 2,630 predicted proteins, 36% of which are unrelated to any previously reported. Analysis of the genome sequence shows the presence of pathways for uptake and utilization of amino acids, active sodium-proton antiporter and potassium uptake systems, sophisticated photosensory and signal transduction pathways, and DNA replication, transcription, and translation systems resembling more complex eukaryotic organisms. Whole proteome comparisons show the definite archaeal nature of this halophile with additional similarities to the Gram-positive Bacillus subtilis and other bacteria. The ease of culturing Halobacterium and the availability of methods for its genetic manipulation in the laboratory, including construction of gene knockouts and replacements, indicate this halophile can serve as an excellent model system among the archaea.
UR - http://www.scopus.com/inward/record.url?scp=12944270421&partnerID=8YFLogxK
U2 - 10.1073/pnas.190337797
DO - 10.1073/pnas.190337797
M3 - Article
C2 - 11016950
AN - SCOPUS:12944270421
SN - 0027-8424
VL - 97
SP - 12176
EP - 12181
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 22
ER -