Genetic transformation of a halophilic archaebacterium with a gas vesicle gene cluster restores its ability to float

John T. Halladay; Ng Wai-lap; Shiladitya DasSarma

doi:10.1016/0378-1119(92)90078-4

Genetic transformation of a halophilic archaebacterium with a gas vesicle gene cluster restores its ability to float

John T. Halladay, Ng Wai-lap, Shiladitya DasSarma^*

^*Corresponding author for this work

Department of Biotechnology and Laboratory Science in Medicine

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The halophilic archaebacterium, Halobacterium halobium, and many other aquatic bacteria synthesize gas-filled vesicles for notation. We recently identified a cluster of 13 genes (gvpMLKJIHGFEDACN) on a 200-kb H. halobium plasmid, pNRC100, involved in gas vesicle synthesis. We have cloned and reconstructed the gvp gene cluster on an H. halobium-E. coli shuttle plasmid. Transformation of H. halobium Vac^- mutants lacking the entire gas vesicle gene region with the gvp gene cluster results in restoration of their ability to float. These results open the way toward further genetic analysis of gas vesicle gene functions and directed flotation of other microorganisms with potential biotechnological applications.

Original language	English
Pages (from-to)	131-136
Number of pages	6
Journal	Gene
Volume	119
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(92)90078-4
State	Published - 21 Sep 1992

Keywords

Biotechnology
Halobacterium halobium, gas vacuoles
archaebacteria
insertion sequences
recombinant DNA
transposition

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10.1016/0378-1119(92)90078-4

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title = "Genetic transformation of a halophilic archaebacterium with a gas vesicle gene cluster restores its ability to float",

abstract = "The halophilic archaebacterium, Halobacterium halobium, and many other aquatic bacteria synthesize gas-filled vesicles for notation. We recently identified a cluster of 13 genes (gvpMLKJIHGFEDACN) on a 200-kb H. halobium plasmid, pNRC100, involved in gas vesicle synthesis. We have cloned and reconstructed the gvp gene cluster on an H. halobium-E. coli shuttle plasmid. Transformation of H. halobium Vac- mutants lacking the entire gas vesicle gene region with the gvp gene cluster results in restoration of their ability to float. These results open the way toward further genetic analysis of gas vesicle gene functions and directed flotation of other microorganisms with potential biotechnological applications.",

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T1 - Genetic transformation of a halophilic archaebacterium with a gas vesicle gene cluster restores its ability to float

AU - Halladay, John T.

AU - Wai-lap, Ng

AU - DasSarma, Shiladitya

PY - 1992/9/21

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N2 - The halophilic archaebacterium, Halobacterium halobium, and many other aquatic bacteria synthesize gas-filled vesicles for notation. We recently identified a cluster of 13 genes (gvpMLKJIHGFEDACN) on a 200-kb H. halobium plasmid, pNRC100, involved in gas vesicle synthesis. We have cloned and reconstructed the gvp gene cluster on an H. halobium-E. coli shuttle plasmid. Transformation of H. halobium Vac- mutants lacking the entire gas vesicle gene region with the gvp gene cluster results in restoration of their ability to float. These results open the way toward further genetic analysis of gas vesicle gene functions and directed flotation of other microorganisms with potential biotechnological applications.

AB - The halophilic archaebacterium, Halobacterium halobium, and many other aquatic bacteria synthesize gas-filled vesicles for notation. We recently identified a cluster of 13 genes (gvpMLKJIHGFEDACN) on a 200-kb H. halobium plasmid, pNRC100, involved in gas vesicle synthesis. We have cloned and reconstructed the gvp gene cluster on an H. halobium-E. coli shuttle plasmid. Transformation of H. halobium Vac- mutants lacking the entire gas vesicle gene region with the gvp gene cluster results in restoration of their ability to float. These results open the way toward further genetic analysis of gas vesicle gene functions and directed flotation of other microorganisms with potential biotechnological applications.

KW - Biotechnology

KW - Halobacterium halobium, gas vacuoles

KW - archaebacteria

KW - insertion sequences

KW - recombinant DNA

KW - transposition

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Genetic transformation of a halophilic archaebacterium with a gas vesicle gene cluster restores its ability to float

Abstract

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