Gal-1 (Galectin-1) Upregulation Contributes to Abdominal Aortic Aneurysm Progression by Enhancing Vascular Inflammation

Ming Tsai Chiang, I. Ming Chen, Fu Fei Hsu, Yen Hui Chen, Min Shao Tsai, Yaw Wen Hsu, Hsin Bang Leu, Po Hsun Huang, Jaw Wen Chen, Fu Tong Liu, Ying Hwa Chen*, Lee Young Chau*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

OBJECTIVE: Abdominal aortic aneurysm (AAA) is a vascular degenerative disease causing sudden rupture of aorta and significant mortality in elders. Nevertheless, no prognostic and therapeutic target is available for disease management. Gal-1 (galectin-1) is a β-galactoside-binding lectin constitutively expressed in vasculature with roles in maintaining vascular homeostasis. This study aims to investigate the potential involvement of Gal-1 in AAA progression. APPROACH AND RESULTS: Gal-1 was significantly elevated in circulation and aortic tissues of Ang II (angiotensin II)-infused apoEdeficient mice developing AAA. Gal-1 deficiency reduced incidence and severity of AAA with lower expression of aortic MMPs (matrix metalloproteases) and proinflammatory cytokines. TNFα (tumor necrosis factor alpha) induced Gal-1 expression in cultured vascular smooth muscle cells and adventitial fibroblasts. Gal-1 deletion enhanced TNFα-induced MMP9 expression in fibroblasts but not vascular smooth muscle cells. Cysteinyl-labeling assay demonstrated that aortic Gal-1 exhibited susceptibility to oxidation in vivo. Recombinant oxidized Gal-1 induced expression of MMP9 and inflammatory cytokines to various extents in macrophages, vascular smooth muscle cells, and fibroblasts through activation of MAP (mitogen-activated protein) kinase signaling. Clinically, serum MMP9 level was significantly higher in both patients with AAA and coronary artery disease than in control subjects, whereas serum Gal-1 level was elevated in patients with AAA but not coronary artery disease when compared with controls. CONCLUSIONS: Gal-1 is highly induced and contributes to AAA by enhancing matrix degradation activity and inflammatory responses in experimental model. The pathological link between Gal-1 and AAA is also observed in human patients.

Original languageEnglish
Pages (from-to)331-345
Number of pages15
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume41
Issue number1
DOIs
StatePublished - Jan 2021

Keywords

  • Abdominal
  • Aorta
  • Aortic aneurysm
  • Cytokines
  • Galectin 1
  • Incidence
  • Matrix metalloprotease

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